{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T22:29:11Z","timestamp":1792794551480,"version":"4.5.0"},"reference-count":30,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2011,4,24]],"date-time":"2011-04-24T00:00:00Z","timestamp":1303603200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2011,6]]},"abstract":"<jats:p>Aquaporins (AQP) play important roles in water and glycerol transport. We examined whether AQP3 is expressed in primary squamous cell carcinoma (SCC) such as esophageal and oral cancer and lymph node metastasis, and whether AQP3 is a potential target for tumor therapy. A high level expression of AQP3 was observed in tumor areas of human primary SCC such as esophageal and lingual cancers, and lymph node metastasis, but was not observed in normal areas. Treatment with pan\u2010AQP inhibitor caused apoptotic cell death on the SCC cell lines in a concentration\u2010dependent manner. Small interfering RNA (siRNA) specific for AQP3 also inhibited cell adhesion and growth of SCC, but not those of adenocarcinoma cell lines and fibroblasts. Expression of integrin \u03b15 and \u03b21, counter adhesion molecules for fibronectin, was inhibited by treatment with AQP3\u2010siRNA. The phosphorylation of focal adhesion kinase (FAK) was decreased by treatment with AQP3\u2010siRNA, which then caused decreases in phosphorylation of Erk and MAPK. These results indicate that the decreases in integrins and the inhibition of cell adhesion might cause inhibition of the FAK signaling pathways. Combination of AQP3\u2010siRNA with cisplatin, a major anti\u2010cancer drug, strongly inhibited the growth of SCC. Cell death caused by the inhibition of AQP3 was a result of direct interference with cell adhesion involving intracellular FAK\u2010MAPK signaling pathways. These results imply a potentially important and novel role for the inhibition of AQP3 function via the use of specific siRNA in the treatment of SCC. (<jats:italic>Cancer Sci<\/jats:italic> 2011; 102: 1128\u20131136)<\/jats:p>","DOI":"10.1111\/j.1349-7006.2011.01927.x","type":"journal-article","created":{"date-parts":[[2011,3,14]],"date-time":"2011-03-14T16:08:35Z","timestamp":1300118915000},"page":"1128-1136","source":"Crossref","is-referenced-by-count":98,"title":["Critical role of aquaporin 3 on growth of human esophageal and oral squamous cell carcinoma"],"prefix":"10.1111","volume":"102","author":[{"given":"Morio","family":"Kusayama","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koichiro","family":"Wada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahide","family":"Nagata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shunsuke","family":"Ishimoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirokazu","family":"Takahashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masato","family":"Yoneda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsushi","family":"Nakajima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaya","family":"Okura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikihiko","family":"Kogo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshinori","family":"Kamisaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,4,24]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-59600-1_20"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1054\/bjom.1999.0031"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.3322\/canjclin.48.4.195"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0142(19980301)82:5<804::AID-CNCR2>3.0.CO;2-J"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.256.5055.385"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature02503"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.02519"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1177\/153537020523000910"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2005.10.081"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.2000.278.1.F13"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0409232102"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-440X(02)00355-X"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1042\/BC20040127"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2006.07.015"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-2042.2007.01901.x"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01482-07"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-008-0303-9"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-05-0087"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2006.00250.x"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2007.00437.x"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1254\/jphs.08339FP"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19920515)69:10<2589::AID-CNCR2820691032>3.0.CO;2-Y"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.13.6161"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/35010517"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.124.4.619"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.28.16995"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02409059"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-0674(97)80155-X"},{"key":"e_1_2_7_30_2","doi-asserted-by":"publisher","DOI":"10.1002\/em.20056"},{"key":"e_1_2_7_31_2","doi-asserted-by":"publisher","DOI":"10.1385\/MO:23:1:1"}],"container-title":["Cancer Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1349-7006.2011.01927.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1349-7006.2011.01927.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,15]],"date-time":"2023-10-15T17:41:37Z","timestamp":1697391697000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1349-7006.2011.01927.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,4,24]]},"references-count":30,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2011,6]]}},"alternative-id":["10.1111\/j.1349-7006.2011.01927.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1349-7006.2011.01927.x","archive":["Portico"],"relation":{},"ISSN":["1347-9032","1349-7006"],"issn-type":[{"value":"1347-9032","type":"print"},{"value":"1349-7006","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,4,24]]}}}