{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T02:28:36Z","timestamp":1791080916967,"version":"4.1.0"},"reference-count":22,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2011,5,9]],"date-time":"2011-05-09T00:00:00Z","timestamp":1304899200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2011,7]]},"abstract":"<jats:p>Adult T\u2010cell leukemia\/lymphoma (ATLL) is a T\u2010cell malignancy associated with HTLV\u20101. The HTLV\u20101 provirus genome has the pX region that encodes <jats:italic>tax<\/jats:italic> and HTLV\u20101 basic leucine zipper factor (<jats:italic>HBZ<\/jats:italic>). Previous studies have reported that the <jats:italic>tax<\/jats:italic> gene is expressed in few ATLL cases, but the <jats:italic>HBZ<\/jats:italic> gene in all ATLL cases. In this study, we used <jats:italic>HBZ<\/jats:italic> gene <jats:italic>in situ<\/jats:italic> hybridization (HBZ\u2010ISH) for detection of the <jats:italic>HBZ<\/jats:italic> gene in formalin\u2010fixed paraffin\u2010embedded tissues. This method showed that all cases (<jats:italic>n<\/jats:italic>\u2003=\u200319) were positive for the ATLL cell line (MT\u20101, MT\u20102, and MT\u20104) and ATLL mouse model (HBZ\u2010Tg mice and NOD\/SCID\/\u03b22\u2010microglobulin<jats:sup>null<\/jats:sup> mice with ATLL transplanted), and the <jats:italic>HBZ<\/jats:italic> gene was also detected in all human ATLL cases (<jats:italic>n<\/jats:italic>\u2003=\u200316). The percentage of positive cells in HBZ\u2010ISH was 5\u201370%. Immunohistochemical staining for Tax protein showed positivity in seven of 11 cases in NOD\/SCID\/\u03b22\u2010microglobulin<jats:sup>null<\/jats:sup> mice with ATLL transplanted and in six of eight human ATLL cases, but the percentage of positive cells was very low (range, 1\u20135%). Although HBZ\u2010ISH is unsuitable to detect HTLV\u20101 clonality, this method is convenient and can be useful for the histological diagnosis of ATLL in HTLV\u20101 sero\u2010indeterminate patients. (<jats:italic>Cancer Sci<\/jats:italic> 2011; 102: 1432\u20131436)<\/jats:p>","DOI":"10.1111\/j.1349-7006.2011.01946.x","type":"journal-article","created":{"date-parts":[[2011,3,31]],"date-time":"2011-03-31T15:41:42Z","timestamp":1301586102000},"page":"1432-1436","source":"Crossref","is-referenced-by-count":18,"title":["Detection of HTLV\u20101 by means of <i>HBZ<\/i> gene <i>in situ<\/i> hybridization in formalin\u2010fixed and paraffin\u2010embedded tissues"],"prefix":"10.1111","volume":"102","author":[{"given":"Kei","family":"Shimizu\u2010Kohno","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yorifumi","family":"Satou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumiko","family":"Arakawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junichi","family":"Kiyasu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshizo","family":"Kimura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisuke","family":"Niino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuo","family":"Sugita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumihiko","family":"Ishikawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masao","family":"Matsuoka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koichi","family":"Ohshima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,5,9]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V50.3.481.481"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.77.12.7415"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.6.2031"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.20007"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0507631103"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1001274"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403829"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1962210"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi960436k"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(01)00303-7"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00196147"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19960328)66:1<18::AID-IJC4>3.0.CO;2-1"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.10.6476"},{"key":"e_1_2_7_15_2","first-page":"978","article-title":"A 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