{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T15:06:24Z","timestamp":1761059184422},"reference-count":23,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2011,5,9]],"date-time":"2011-05-09T00:00:00Z","timestamp":1304899200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2011,7]]},"abstract":"<jats:p>Partial human leukocyte antigen (HLA)\u2010mismatched hematopoietic stem cell transplantation (HSCT) is often performed when an HLA\u2010matched donor is not available. In these cases, CD8<jats:sup>+<\/jats:sup> or CD4<jats:sup>+<\/jats:sup> T cell responses are induced depending on the mismatched HLA class I or II allele(s). Herein, we report on an HLA\u2010DRB1*08:03\u2010restricted CD8<jats:sup>+<\/jats:sup> CTL clone, named CTL\u20101H8, isolated from a patient following an HLA\u2010DR\u2010mismatched HSCT from his brother. Lysis of a patient Epstein\u2013Barr virus\u2010transformed B cell line (B\u2010LCL) by CTL\u20101H8 was inhibited after the addition of blocking antibodies against HLA\u2010DR and CD8, whereas antibodies against pan\u2010HLA class I or CD4 had no effect. The 1H8\u2010CTL clone did not lyse the recipient dermal fibroblasts whose HLA\u2010DRB1*08:03 expression was upregulated after 1\u2003week cytokine treatment. Engraftment of HLA\u2010DRB1*08:03\u2010positive primary leukemic stem cells in non\u2010obese diabetic\/severe combined immunodeficient\/\u03b3c\u2010null (NOG) mice was completely inhibited by the <jats:italic>in vitro<\/jats:italic> preincubation of cells with CTL\u20101H8, suggesting that HLA\u2010DRB1*08:03 is expressed on leukemic stem cells. Finally, analysis of the precursor frequency of CD8<jats:sup>+<\/jats:sup> CTL specific for recipient antigens in post\u2010HSCT peripheral blood T cells revealed a significant fraction of the total donor CTL responses towards the individual mismatched HLA\u2010DR antigen in two patients. These findings underscore unexpectedly significant CD8 T cell responses in the context of HLA class II. (<jats:italic>Cancer Sci<\/jats:italic> 2011; 102: 1281\u20131286)<\/jats:p>","DOI":"10.1111\/j.1349-7006.2011.01949.x","type":"journal-article","created":{"date-parts":[[2011,4,5]],"date-time":"2011-04-05T17:36:23Z","timestamp":1302024983000},"page":"1281-1286","source":"Crossref","is-referenced-by-count":7,"title":["Mismatched human leukocyte antigen class II\u2010restricted CD8<sup>+<\/sup> cytotoxic T cells may mediate selective graft\u2010versus\u2010leukemia effects following allogeneic hematopoietic cell 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