{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T22:05:34Z","timestamp":1762898734757,"version":"3.33.0"},"reference-count":17,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2011,11,28]],"date-time":"2011-11-28T00:00:00Z","timestamp":1322438400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2012,2]]},"abstract":"<jats:p>Whole genome\u2010scale integrated analyses of exon array and array\u2010comparative genomic hybridization are expected to enable the identification of unknown genetic features of cancer cells. Here, we evaluated this approach in 22 gastric and colorectal cancer cell lines, focusing on protein kinase genes and genes belonging to the cadherin\u2013catenin family. Regarding alternative splicing patterns, several cancer cell lines predominantly expressed isoform 1 of protein kinase A catalytic subunit beta (<jats:italic>PRKACB<\/jats:italic>). Paired gastric cancer specimens demonstrated that isoform 1 of <jats:italic>PRKACB<\/jats:italic> was a novel cancer\u2010related variant transcript in gastric cancers. In addition, the exon array analysis clearly identified exon 3 or exon 3\u20134 skipping in <jats:italic>catenin beta 1<\/jats:italic>, a short intron insertion with exon 9 skipping in <jats:italic>CDH1<\/jats:italic>, and a deletional transcript of <jats:italic>CDH13<\/jats:italic>. These abnormal transcripts were shown to have arisen from small genomic deletions. Meanwhile, an integrated analysis of 11 gastric cancer cell lines revealed that four cell lines amplified fibroblast growth factor receptor 2, with truncated forms observed in two of the cell lines. Gene amplification, and not the truncated form, was found to determine the sensitivity to a fibroblast growth factor receptor inhibitor, indicating that our cell line panel might be useful for cell\u2010based evaluations of specific inhibitors. Using an integrated analysis, we identified several abnormal transcripts and genomic alterations in gastric and colorectal cancer cells. Our approach might enable genetic changes to be identified more efficiently, and the present results warrant further investigation using clinical samples and integrated analyses. (<jats:italic>Cancer Sci<\/jats:italic> 2012; 103: 221\u2013227)<\/jats:p>","DOI":"10.1111\/j.1349-7006.2011.02132.x","type":"journal-article","created":{"date-parts":[[2011,10,28]],"date-time":"2011-10-28T17:31:50Z","timestamp":1319823110000},"page":"221-227","source":"Crossref","is-referenced-by-count":19,"title":["Integrated analysis of whole genome exon array and array\u2010comparative genomic hybridization in gastric and colorectal cancer cells"],"prefix":"10.1111","volume":"103","author":[{"given":"Kazuyuki","family":"Furuta","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tokuzo","family":"Arao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuko","family":"Sakai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hideharu","family":"Kimura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoyuki","family":"Nagai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daisuke","family":"Tamura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keiichi","family":"Aomatsu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kanae","family":"Kudo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyasu","family":"Kaneda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshihiko","family":"Fujita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuko","family":"Matsumoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuhide","family":"Yamada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuyoshi","family":"Yanagihara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masaru","family":"Sekijima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuto","family":"Nishio","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,11,28]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_7_2_2","DOI":"10.1093\/nar\/gkn697"},{"doi-asserted-by":"publisher","key":"e_1_2_7_3_2","DOI":"10.1186\/1471-2164-9-216"},{"doi-asserted-by":"publisher","key":"e_1_2_7_4_2","DOI":"10.1371\/journal.pone.0002392"},{"doi-asserted-by":"publisher","key":"e_1_2_7_5_2","DOI":"10.1186\/1471-2164-7-325"},{"doi-asserted-by":"publisher","key":"e_1_2_7_6_2","DOI":"10.1016\/j.drudis.2008.06.007"},{"doi-asserted-by":"publisher","key":"e_1_2_7_7_2","DOI":"10.1016\/S1470-2045(07)70104-3"},{"doi-asserted-by":"publisher","key":"e_1_2_7_8_2","DOI":"10.1158\/0008-5472.CAN-04-2360"},{"doi-asserted-by":"publisher","key":"e_1_2_7_9_2","DOI":"10.1158\/0008-5472.CAN-09-2161"},{"doi-asserted-by":"publisher","key":"e_1_2_7_10_2","DOI":"10.1016\/j.cellsig.2008.02.022"},{"doi-asserted-by":"publisher","key":"e_1_2_7_11_2","DOI":"10.1046\/j.0014-2956.2001.02429.x"},{"doi-asserted-by":"publisher","key":"e_1_2_7_12_2","DOI":"10.1126\/scisignal.122re4"},{"doi-asserted-by":"publisher","key":"e_1_2_7_13_2","DOI":"10.1093\/emboj\/cdg177"},{"doi-asserted-by":"publisher","key":"e_1_2_7_14_2","DOI":"10.1136\/jmg.2004.018275"},{"doi-asserted-by":"publisher","key":"e_1_2_7_15_2","DOI":"10.1371\/journal.pone.0007887"},{"doi-asserted-by":"publisher","key":"e_1_2_7_16_2","DOI":"10.1158\/1078-0432.CCR-06-2743"},{"doi-asserted-by":"publisher","key":"e_1_2_7_17_2","DOI":"10.1002\/cncr.21409"},{"doi-asserted-by":"publisher","key":"e_1_2_7_18_2","DOI":"10.1016\/j.ceb.2009.01.012"}],"container-title":["Cancer Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1349-7006.2011.02132.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1349-7006.2011.02132.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,24]],"date-time":"2025-01-24T12:27:46Z","timestamp":1737721666000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1349-7006.2011.02132.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,11,28]]},"references-count":17,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2012,2]]}},"alternative-id":["10.1111\/j.1349-7006.2011.02132.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1349-7006.2011.02132.x","archive":["Portico"],"relation":{},"ISSN":["1347-9032","1349-7006"],"issn-type":[{"type":"print","value":"1347-9032"},{"type":"electronic","value":"1349-7006"}],"subject":[],"published":{"date-parts":[[2011,11,28]]}}}