{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T18:09:50Z","timestamp":1793642990230,"version":"4.5.1"},"reference-count":47,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2012,5,15]],"date-time":"2012-05-15T00:00:00Z","timestamp":1337040000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cancer Science"],"published-print":{"date-parts":[[2012,8]]},"abstract":"E<\/jats:styled-content>pstein\u2013B<\/jats:styled-content>arr virus (EBV<\/jats:styled-content>) infects various types of lymphocytes and is associated with not only B<\/jats:styled-content> cell\u2010origin lymphoma, but also T<\/jats:styled-content> or natural killer cell lymphoproliferative diseases (T<\/jats:styled-content>\/NK LPD<\/jats:styled-content>). Recently, we established a novel assay to identify EBV<\/jats:styled-content>\u2010infected cells using FISH<\/jats:styled-content>. Using this assay, dual staining with antibodies to both surface antigens and an EBV<\/jats:styled-content>\u2010encoded small RNA<\/jats:styled-content> (EBER<\/jats:styled-content>) probe can be performed. In the present study, we applied this recently developed FISH<\/jats:styled-content> assay to EBV<\/jats:styled-content>\u2010associated T<\/jats:styled-content>\/NK LPD<\/jats:styled-content> to confirm its diagnostic utility. Using FISH<\/jats:styled-content>, we prospectively analyzed peripheral blood from patients with suspected EBV<\/jats:styled-content>\u2010associated T<\/jats:styled-content>\/NK LPD<\/jats:styled-content>. The results were compared with those obtained using immunobead sorting followed by quantitative PCR<\/jats:styled-content>. In all, 26 patients were included study. Using FISH<\/jats:styled-content>, 0.15\u201367.0% of peripheral blood lymphocytes were found to be positive for EBER<\/jats:styled-content>. Dual staining was used to determine EBER<\/jats:styled-content>\u2010positive cell phenotypes in 23 of 26 subjects (88.5%). In five of seven patients with hydroa vacciniforme\u2010like lymphoma (an EBV<\/jats:styled-content>\u2010positive cutaneous T<\/jats:styled-content> cell lymphoma), EBER<\/jats:styled-content>\u2010positive cells were identified as CD<\/jats:styled-content>3+<\/jats:sup>CD<\/jats:styled-content>4\u2212<\/jats:sup>CD<\/jats:styled-content>8\u2212\u00a0<\/jats:sup>TCR<\/jats:styled-content>\u03b3\u03b4+<\/jats:sup> T cells. Furthermore, in a 25\u2010year\u2010old male patient with systemic EBV<\/jats:styled-content>\u2010positive T<\/jats:styled-content> cell LPD<\/jats:styled-content>, two lymphocyte lineages were positive for EBER<\/jats:styled-content>: CD<\/jats:styled-content>4+<\/jats:sup>CD<\/jats:styled-content>8\u2212<\/jats:sup> and CD<\/jats:styled-content>4\u2212<\/jats:sup>CD<\/jats:styled-content>8+<\/jats:sup>T<\/jats:styled-content> cells. Thus, we confirmed that our newly developed assay is useful for quantifying and characterizing EBV<\/jats:styled-content>\u2010infected lymphocytes in EBV<\/jats:styled-content>\u2010associated T<\/jats:styled-content>\/NK LPD<\/jats:styled-content> and that it can be used not only to complement the pathological diagnosis, but also to clarify the pathogenesis and to expand the spectrum of EBV<\/jats:styled-content>\u2010associated diseases. (Cancer Sci,<\/jats:italic> doi: 10.1111\/j.1349\u20107006.2012.02305.x, 2012)<\/jats:p>","DOI":"10.1111\/j.1349-7006.2012.02305.x","type":"journal-article","created":{"date-parts":[[2012,4,12]],"date-time":"2012-04-12T14:10:28Z","timestamp":1334239828000},"page":"1481-1488","source":"Crossref","is-referenced-by-count":31,"title":["Application of flow cytometric in situ<\/i> hybridization assay to E<\/scp>pstein\u2013B<\/scp>arr virus\u2010associated T<\/scp>\/natural killer cell lymphoproliferative diseases"],"prefix":"10.1111","volume":"103","author":[{"given":"Shinji","family":"Kawabe","sequence":"first","affiliation":[{"name":"Department of Pediatrics Nagoya University Graduate School of Medicine Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshinori","family":"Ito","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Nagoya University Graduate School of Medicine Nagoya 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