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Gene\u2010specific probes could be designed for all genes of\n                    <jats:italic>Pectobacterium atrosepticum<\/jats:italic>\n                    ,\n                    <jats:italic>c.<\/jats:italic>\n                    \u00a050% of the genes of\n                    <jats:italic>Streptomyces scabies<\/jats:italic>\n                    and\n                    <jats:italic>c.<\/jats:italic>\n                    \u00a030% of the genes of\n                    <jats:italic>Clavibacter michiganensis<\/jats:italic>\n                    ssp.\n                    <jats:italic>sepedonicus<\/jats:italic>\n                    utilizing the whole\u2010genome sequence information available. For\n                    <jats:italic>Streptomyces turgidiscabies<\/jats:italic>\n                    , 226 probes were designed according to the sequences of a pathogenicity island containing important virulence genes. In addition, probes were designed for the virulence\u2010associated\n                    <jats:italic>nip<\/jats:italic>\n                    (necrosis\u2010inducing protein) genes of\n                    <jats:italic>P<\/jats:italic>\n                    .\n                    <jats:italic>\u00a0atrosepticum<\/jats:italic>\n                    ,\n                    <jats:italic>P<\/jats:italic>\n                    .\n                    <jats:italic>\u00a0carotovorum<\/jats:italic>\n                    and\n                    <jats:italic>Dickeya dadantii<\/jats:italic>\n                    and for the intergenic spacer (IGS) sequences of the 16S\u201323S rRNA gene region.\n                    <jats:italic>Ralstonia solanacearum<\/jats:italic>\n                    was not included in the study, because it is a quarantine organism and is not presently found in Finland, but a few probes were also designed for this species. The probes contained on average 40 target\u2010specific nucleotides and were synthesized on the array\n                    <jats:italic>in situ<\/jats:italic>\n                    , organized as eight sub\u2010arrays with an identical set of probes which could be used for hybridization with different samples. All bacteria were readily distinguished using a single channel system for signal detection. Nearly all of the\n                    <jats:italic>c.<\/jats:italic>\n                    \u00a01000 probes designed for\n                    <jats:italic>C<\/jats:italic>\n                    .\n                    <jats:italic>\u00a0michiganensis<\/jats:italic>\n                    ssp.\n                    <jats:italic>sepedonicus<\/jats:italic>\n                    ,\n                    <jats:italic>c.<\/jats:italic>\n                    \u00a050% and 40% of the\n                    <jats:italic>c.<\/jats:italic>\n                    \u00a04000 probes designed for the genes of\n                    <jats:italic>S<\/jats:italic>\n                    .\n                    <jats:italic>\u00a0scabies<\/jats:italic>\n                    and\n                    <jats:italic>P<\/jats:italic>\n                    .\n                    <jats:italic>\u00a0atrosepticum<\/jats:italic>\n                    , respectively, and over 100 probes for\n                    <jats:italic>S.\u00a0turgidiscabies<\/jats:italic>\n                    showed significant signals only with the respective species.\n                    <jats:italic>P.\u00a0atrosepticum<\/jats:italic>\n                    ,\n                    <jats:italic>P.\u00a0carotovorum<\/jats:italic>\n                    and\n                    <jats:italic>Dickeya<\/jats:italic>\n                    strains were all detected with 110 common probes. By contrast, the strains of these species were found to differ in their signal profiles. Probes targeting the IGS region and\n                    <jats:italic>nip<\/jats:italic>\n                    genes could be used to place strains of\n                    <jats:italic>Dickeya<\/jats:italic>\n                    to two groups, which correlated with differences in virulence. Taken together, the approach of using a custom\u2010designed, genome\u2010wide microarray provided a robust means for distinguishing the bacterial pathogens of potato.\n                  <\/jats:p>","DOI":"10.1111\/j.1364-3703.2008.00482.x","type":"journal-article","created":{"date-parts":[[2008,5,23]],"date-time":"2008-05-23T03:45:34Z","timestamp":1211514334000},"page":"705-717","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Distinguishing bacterial pathogens of potato using a genome\u2010wide microarray approach"],"prefix":"10.1111","volume":"9","author":[{"given":"M.","family":"AITTAMAA","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"SOMERVUO","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"PIRHONEN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"MATTINEN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"NISSINEN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"AUVINEN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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