{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T08:17:51Z","timestamp":1791188271145,"version":"4.1.0"},"reference-count":57,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2011,8,7]],"date-time":"2011-08-07T00:00:00Z","timestamp":1312675200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2012,2]]},"abstract":"<jats:title>SUMMARY<\/jats:title>\n                  <jats:p>\n                    The receptor\u2010like protein kinases (RLKs) constitute a large and diverse group of proteins controlling numerous plant physiological processes, including development, hormone perception and stress responses. The cysteine\u2010rich RLKs (CRKs) represent a prominent subfamily of transmembrane\u2010anchored RLKs. We have identified a putative barley (\n                    <jats:italic>Hordeum vulgare<\/jats:italic>\n                    ) CRK gene family member, designated\n                    <jats:italic>HvCRK1<\/jats:italic>\n                    . The mature putative protein comprises 645 amino acids, and includes a putative receptor domain containing two characteristic \u2018domain 26 of unknown function\u2019 (duf26) domains in the N\u2010terminal region, followed by a rather short 17\u2010amino\u2010acid transmembrane domain, which includes an AAA motif, two features characteristic of endoplasmic reticulum (ER)\u2010targeted proteins and, finally, a characteristic putative protein kinase domain in the C\u2010terminus. The\n                    <jats:italic>HvCRK1<\/jats:italic>\n                    transcript was isolated from leaves inoculated with the biotrophic fungal pathogen\n                    <jats:italic>Blumeria graminis<\/jats:italic>\n                    f.sp.\n                    <jats:italic>hordei<\/jats:italic>\n                    (\n                    <jats:italic>Bgh<\/jats:italic>\n                    ).\n                    <jats:italic>HvCRK1<\/jats:italic>\n                    transcripts were observed to accumulate transiently following\n                    <jats:italic>Bgh<\/jats:italic>\n                    inoculation of susceptible barley. Transient silencing of\n                    <jats:italic>HvCRK1<\/jats:italic>\n                    expression in bombarded epidermal cells led to enhanced resistance to\n                    <jats:italic>Bgh<\/jats:italic>\n                    , but did not affect\n                    <jats:italic>R<\/jats:italic>\n                    \u2010gene\u2010mediated resistance. Silencing of\n                    <jats:italic>HvCRK1<\/jats:italic>\n                    phenocopied the effective penetration resistance found in\n                    <jats:italic>mlo<\/jats:italic>\n                    \u2010resistant barley plants, and the possible link between\n                    <jats:italic>Hv<\/jats:italic>\n                    CRK1 and MLO was substantiated by the fact that\n                    <jats:italic>HvCRK1<\/jats:italic>\n                    induction on\n                    <jats:italic>Bgh<\/jats:italic>\n                    inoculation was dependent on\n                    <jats:italic>Mlo<\/jats:italic>\n                    . Finally, using both experimental and\n                    <jats:italic>in\u2003silico<\/jats:italic>\n                    approaches, we demonstrated that\n                    <jats:italic>Hv<\/jats:italic>\n                    CRK1 localizes to the ER of barley cells. The negative effect on basal resistance against\n                    <jats:italic>Bgh<\/jats:italic>\n                    and the functional aspects of MLO\u2010 and ER\u2010localized\n                    <jats:italic>Hv<\/jats:italic>\n                    CRK1 signalling on\n                    <jats:italic>Bgh<\/jats:italic>\n                    inoculation are discussed.\n                  <\/jats:p>","DOI":"10.1111\/j.1364-3703.2011.00736.x","type":"journal-article","created":{"date-parts":[[2011,8,8]],"date-time":"2011-08-08T00:03:25Z","timestamp":1312761805000},"page":"135-147","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":66,"title":["Regulation of basal resistance by a powdery mildew\u2010induced cysteine\u2010rich receptor\u2010like protein kinase in barley"],"prefix":"10.1111","volume":"13","author":[{"given":"CBGOWDA","family":"RAYAPURAM","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MICHAEL K.","family":"JENSEN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"FABIAN","family":"MAISER","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JOHANNA VIKGREN","family":"SHANIR","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"HENRIK","family":"HORNSH\u00d8J","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"JESPER H.","family":"RUNG","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"PER L.","family":"GREGERSEN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"PATRICK","family":"SCHWEIZER","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"DAVID B.","family":"COLLINGE","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MICHAEL F.","family":"LYNGKJ\u00c6R","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,8,7]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2007.03064.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0500012102"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.000620"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8362242"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81912-1"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0885-5765(05)80028-1"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:PLAN.0000009265.72567.58"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11103-004-3381-2"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.126.2.473"},{"key":"e_1_2_6_11_1","first-page":"146","volume-title":"The Powdery Mildews: A Comprehensive Treatise","author":"Collinge D.B.","year":"2002"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.49.34993"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.00998"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.106.046326"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-18-0755"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2000.00923.x"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006367609556"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2005.02617.x"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5266(00)00177-1"},{"key":"e_1_2_6_20_1","first-page":"257","article-title":"Penetration attempts by the powdery mildew fungus into barley leaves are accompanied by increased gene transcript accumulation in the epidermal cell layer. 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