{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T11:04:15Z","timestamp":1789988655174,"version":"4.0.1"},"reference-count":46,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,9,26]],"date-time":"2012-09-26T00:00:00Z","timestamp":1348617600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["bsppjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Molecular Plant Pathology"],"published-print":{"date-parts":[[2013,2]]},"abstract":"Summary<\/jats:title>\n \n \n S<\/jats:styled-content>\n treptomyces scabies\n <\/jats:italic>\n is a model organism for the investigation of plant\u2013microbe interactions in\n G<\/jats:styled-content>\n ram\u2010positive bacteria. Here, we investigate the type\n VII<\/jats:styled-content>\n protein secretion system (\n T<\/jats:styled-content>\n 7\n SS<\/jats:styled-content>\n ) in\n \n S<\/jats:styled-content>\n .\u2009scabies\n <\/jats:italic>\n ; the\n T<\/jats:styled-content>\n 7\n SS<\/jats:styled-content>\n is required for the virulence of other\n G<\/jats:styled-content>\n ram\u2010positive bacteria, including\n \n M<\/jats:styled-content>\n ycobacterium tuberculosis\n <\/jats:italic>\n and\n \n S<\/jats:styled-content>\n taphylococcus aureus\n <\/jats:italic>\n . The hallmarks of a functional\n T<\/jats:styled-content>\n 7\n SS<\/jats:styled-content>\n are an\n E<\/jats:styled-content>\n cc\n C<\/jats:styled-content>\n protein that forms an essential component of the secretion apparatus and two small, sequence\u2010related substrate proteins,\n E<\/jats:styled-content>\n sx\n A<\/jats:styled-content>\n and\n E<\/jats:styled-content>\n sx\n B<\/jats:styled-content>\n . A putative transmembrane protein,\n E<\/jats:styled-content>\n cc\n D<\/jats:styled-content>\n , may also be associated with\n T7S<\/jats:styled-content>\n in Actinobacteria. In this study, we constructed strains of the plant pathogen\n \n S<\/jats:styled-content>\n .\u2009scabies\n <\/jats:italic>\n carrying marked mutations in genes coding for\n E<\/jats:styled-content>\n cc\n C<\/jats:styled-content>\n ,\n E<\/jats:styled-content>\n cc\n D<\/jats:styled-content>\n ,\n E<\/jats:styled-content>\n sx\n A<\/jats:styled-content>\n and\n E<\/jats:styled-content>\n sx\n B<\/jats:styled-content>\n . Unexpectedly, we showed that all four mutant strains retain full virulence towards several plant hosts. However, disruption of the\n \n esx\n A<\/jats:styled-content>\n <\/jats:italic>\n or\n \n esx\n B<\/jats:styled-content>\n <\/jats:italic>\n , but not\n \n ecc\n C<\/jats:styled-content>\n <\/jats:italic>\n or\n \n ecc\n D<\/jats:styled-content>\n <\/jats:italic>\n , genes affects\n \n S<\/jats:styled-content>\n .\u2009scabies\n <\/jats:italic>\n development, including a delay in sporulation, abnormal spore chains and resistance to lysis by the\n \n S<\/jats:styled-content>\n treptomyces\n <\/jats:italic>\n \u2010specific phage \u03d5\n C<\/jats:styled-content>\n 31. We further showed that these phenotypes are specific to the loss of the\n T<\/jats:styled-content>\n 7\n SS<\/jats:styled-content>\n substrate proteins\n E<\/jats:styled-content>\n sx\n A<\/jats:styled-content>\n and\n E<\/jats:styled-content>\n sx\n B<\/jats:styled-content>\n , and are not observed when components of the\n T<\/jats:styled-content>\n 7\n SS<\/jats:styled-content>\n secretion machinery are lacking. Taken together, these results imply an unexpected intracellular role for\n E<\/jats:styled-content>\n sx\n A<\/jats:styled-content>\n and\n E<\/jats:styled-content>\n sx\n B<\/jats:styled-content>\n .\n <\/jats:p>","DOI":"10.1111\/j.1364-3703.2012.00835.x","type":"journal-article","created":{"date-parts":[[2012,9,26]],"date-time":"2012-09-26T11:07:52Z","timestamp":1348657672000},"page":"119-130","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["The\n ESX<\/scp>\n \/type\n VII<\/scp>\n secretion system modulates development, but not virulence, of the plant pathogen\n \n S<\/scp>\n treptomyces scabies\n <\/i>"],"prefix":"10.1111","volume":"14","author":[{"given":"Joanna K.","family":"Fyans","sequence":"first","affiliation":[{"name":"Division of Molecular Microbiology, College of Life Sciences University of Dundee Dundee DD1\u20095EH UK"},{"name":"James Hutton Institute Invergowrie Dundee DD2\u20095DA UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dawn","family":"Bignell","sequence":"additional","affiliation":[{"name":"Department of Biology Memorial University St. John's NL A1B\u20093X9 Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosemary","family":"Loria","sequence":"additional","affiliation":[{"name":"Plant Pathology Department University of Florida PO Box 110680 Gainesville FL 32611\u20100680 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian","family":"Toth","sequence":"additional","affiliation":[{"name":"James Hutton Institute Invergowrie Dundee DD2\u20095DA UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tracy","family":"Palmer","sequence":"additional","affiliation":[{"name":"Division of Molecular Microbiology, College of Life Sciences University of Dundee Dundee DD1\u20095EH UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,9,26]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.037069-0"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.00023-09"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-23-2-0161"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/ern344"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000507"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.1.88-98.2006"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.179.24.7776-7783.1997"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0405620102"},{"key":"e_1_2_6_10_1","first-page":"1632","volume":"313","author":"Champion P.A.","year":"2006","journal-title":"C\u2010terminal signal sequence promotes virulence factor secretion in. 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