{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T00:53:36Z","timestamp":1697417616756},"reference-count":25,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2004,8,26]],"date-time":"2004-08-26T00:00:00Z","timestamp":1093478400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Physiologica Scandinavica"],"published-print":{"date-parts":[[2004,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Aim:\u2002<\/jats:bold> Acetylcholine (ACh) stimulates ion secretion in the small intestine and colon. The purpose of the present study was to characterize the ACh\u2010induced electrogenic ion transport in human duodenum and determine the muscarinic receptor subtypes functionally involved.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold> Biopsies from the second part of duodenum were obtained from 28 patients during endoscopy. Biopsies were mounted in modified Ussing chambers with air\u2010suction for measurements of short\u2010circuit current by a previously validated technique. Short\u2010circuit current was measured after application of chloride\/bicarbonate transport inhibitors bumetanide, 4\u2010acetamido\u20104\u2032\u2010isothiocyanostilbene\u20102,2\u2032\u2010disulfonic acid (SITS), diphenylamine\u20102\u2010carboxylate (DPC), and acetazolamide. 4\u2010diphenylacetoxy\u2010<jats:italic>N<\/jats:italic>\u2010methylpiperidine methiodide (4\u2010DAMP) and two mamba toxins MT3 and MT7 were used to characterize the mAChR receptor subtypes involved. The effects of transport inhibitors and receptor antagonists were measured by comparing two consecutive responses of ACh on short\u2010circuit current in the same biopsy specimen.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> Bumetanide and 4\u2010DAMP significantly inhibited ACh\u2010induced short\u2010circuit current, whereas SITS, DPC, acetazolamide, mamba toxin MT3, and mamba toxin MT7 all failed to show any significant effect.<\/jats:p><jats:p><jats:bold>Conclusion:\u2002<\/jats:bold> In conclusion, our results indicate that muscarinic receptor subtype M<jats:sub>3<\/jats:sub> acts as the main mediator of bumetanide\u2010sensitive ACh\u2010induced secretion in human duodenum.<\/jats:p>","DOI":"10.1111\/j.1365-201x.2004.01301.x","type":"journal-article","created":{"date-parts":[[2004,8,26]],"date-time":"2004-08-26T08:41:59Z","timestamp":1093509719000},"page":"63-68","source":"Crossref","is-referenced-by-count":4,"title":["Functional characterization of muscarinic receptor subtypes in human duodenal secretion"],"prefix":"10.1111","volume":"182","author":[{"given":"R.","family":"Larsen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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