{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,12,22]],"date-time":"2024-12-22T21:40:15Z","timestamp":1734903615720,"version":"3.32.0"},"reference-count":20,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2005,1,27]],"date-time":"2005-01-27T00:00:00Z","timestamp":1106784000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Physiologica Scandinavica"],"published-print":{"date-parts":[[2005,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Aim:\u2002<\/jats:bold>To evaluate the source and role of acid\u2010induced intraluminal nitric oxide (NO) production in the oesophagus by studying how the exposure of the oesophagus to acid affects NO release, via the NO\u2010producing enzyme NO synthase and its relation to changes in epithelial barrier integrity.<\/jats:p><jats:p><jats:bold>Methods:\u2002<\/jats:bold>Ferrets were anaesthetized and their oesophagi were divided at both ends. The test subjects were pre\u2010treated with the intravenous NO synthase inhibitors<jats:italic>N<\/jats:italic><jats:sup>G<\/jats:sup>\u2010nitro\u2010<jats:sc>l<\/jats:sc>\u2010arginine\u2010methyl ester (<jats:sc>l<\/jats:sc>\u2010NAME, 100\u2003mg\u2003kg<jats:sup>\u22121<\/jats:sup>) and 1400W (12\u2003mg\u2003kg<jats:sup>\u22121<\/jats:sup>). Untreated and<jats:italic>N<\/jats:italic><jats:sup>G<\/jats:sup>\u2010nitro\u2010<jats:sc>d<\/jats:sc>\u2010arginine\u2010methyl ester pre\u2010treated (<jats:sc>d<\/jats:sc>\u2010NAME, 100\u2003mg\u2003kg<jats:sup>\u22121<\/jats:sup>) animals served as controls. The oesophagus was then perfused with either HCl (0.1\u2003<jats:sc>m<\/jats:sc>) or physiological saline for 20\u2003min. The intraluminal NO concentration was determined before and after the acid\/saline infusion while the transmucosal potential difference (PD) was monitored continuously. Oesophageal biopsies were examined for expression of inducible NO synthase using immunohistochemistry.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold>The intraluminal NO concentration increased after acid exposure. This was blocked by<jats:sc>l<\/jats:sc>\u2010NAME and 1400W, but not by<jats:sc>d<\/jats:sc>\u2010NAME. The peak PD response was not affected by agents affecting NO synthesis, while the plateau response was attenuated by<jats:sc>l<\/jats:sc>\u2010NAME,<jats:sc>d<\/jats:sc>\u2010NAME and 1400W. Immunohistochemistry revealed inducible NO synthase expression in the epithelium.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold>Exposing the ferret oesophageal mucosa to acid elicited an increase in juxtamucosal NO formation through the activation of inducible NO synthase. The corresponding electrophysiological observations suggested an association between mucosal NO production and epithelial integrity.<\/jats:p>","DOI":"10.1111\/j.1365-201x.2004.01386.x","type":"journal-article","created":{"date-parts":[[2005,1,27]],"date-time":"2005-01-27T17:13:20Z","timestamp":1106846000000},"page":"211-218","source":"Crossref","is-referenced-by-count":1,"title":["Epithelial barrier integrity and intraluminal nitric oxide production in response to acid perfusion of the ferret oesophagus"],"prefix":"10.1111","volume":"183","author":[{"given":"M.","family":"Bove","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Ruth","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Lundell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Ny","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,1,27]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_16_2_1","DOI":"10.1016\/j.lfs.2003.10.042"},{"key":"e_1_2_16_3_1","first-page":"G1039","article-title":"Continuous measurement of gastric nitric oxide production","volume":"271","author":"Aneman A.","year":"1996","journal-title":"Am J Physiol"},{"key":"e_1_2_16_4_1","doi-asserted-by":"crossref","first-page":"47","DOI":"10.33549\/physiolres.930000.49.47","article-title":"Long\u2010term administration of d\u2010NAME induces hemodynamic and structural changes in the cardiovascular system","volume":"49","author":"Babal P.","year":"2000","journal-title":"Physiol Res"},{"doi-asserted-by":"publisher","key":"e_1_2_16_5_1","DOI":"10.1038\/368502a0"},{"doi-asserted-by":"publisher","key":"e_1_2_16_6_1","DOI":"10.1159\/000073225"},{"doi-asserted-by":"publisher","key":"e_1_2_16_7_1","DOI":"10.1080\/003655299750026155"},{"doi-asserted-by":"publisher","key":"e_1_2_16_8_1","DOI":"10.1080\/00365520212497"},{"doi-asserted-by":"publisher","key":"e_1_2_16_9_1","DOI":"10.1016\/S1590-8658(02)80037-8"},{"key":"e_1_2_16_10_1","first-page":"G1012","article-title":"Nitric oxide: mediator of NANC hyperpolarization of opossum esophageal smooth muscle","volume":"261","author":"Du C.","year":"1991","journal-title":"Am J Physiol"},{"doi-asserted-by":"publisher","key":"e_1_2_16_11_1","DOI":"10.1007\/s005350050178"},{"doi-asserted-by":"publisher","key":"e_1_2_16_12_1","DOI":"10.1007\/978-1-4757-3235-1_3"},{"doi-asserted-by":"publisher","key":"e_1_2_16_13_1","DOI":"10.1023\/A:1015391002252"},{"doi-asserted-by":"publisher","key":"e_1_2_16_14_1","DOI":"10.1159\/000171325"},{"doi-asserted-by":"publisher","key":"e_1_2_16_15_1","DOI":"10.1136\/gut.35.11.1543"},{"key":"e_1_2_16_16_1","first-page":"G59","article-title":"Nitric oxide pathway in cat esophagus: localization of nitric oxide synthase and functional effects","volume":"268","author":"Ny L.","year":"1995","journal-title":"Am J Physiol"},{"key":"e_1_2_16_17_1","first-page":"G718","article-title":"Mechanisms of H+ injury in rabbit esophageal epithelium","volume":"246","author":"Orlando R.C.","year":"1984","journal-title":"Am J Physiol"},{"doi-asserted-by":"publisher","key":"e_1_2_16_18_1","DOI":"10.1007\/s005350200169"},{"doi-asserted-by":"publisher","key":"e_1_2_16_19_1","DOI":"10.1046\/j.1365-201x.2000.00678.x"},{"key":"e_1_2_16_20_1","first-page":"211","article-title":"The effect of nitric oxide donors and l\u2010arginine on the gastric electrolyte barrier","volume":"52","author":"Szlachcic A.","year":"2001","journal-title":"J Physiol Pharmacol"},{"doi-asserted-by":"publisher","key":"e_1_2_16_21_1","DOI":"10.1016\/0016-5085(91)90070-2"}],"container-title":["Acta Physiologica Scandinavica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-201X.2004.01386.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-201X.2004.01386.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,22]],"date-time":"2024-12-22T21:26:04Z","timestamp":1734902764000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-201X.2004.01386.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,1,27]]},"references-count":20,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2005,2]]}},"alternative-id":["10.1111\/j.1365-201X.2004.01386.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-201x.2004.01386.x","archive":["Portico"],"relation":{},"ISSN":["0001-6772","1365-201X"],"issn-type":[{"type":"print","value":"0001-6772"},{"type":"electronic","value":"1365-201X"}],"subject":[],"published":{"date-parts":[[2005,1,27]]}}}