{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,20]],"date-time":"2025-07-20T04:11:41Z","timestamp":1752984701984},"reference-count":28,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2004,9,1]],"date-time":"2004-09-01T00:00:00Z","timestamp":1093996800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Aliment Pharmacol Ther"],"published-print":{"date-parts":[[2004,9]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:bold>Background<\/jats:bold> :\u2002We previously reported that aspirin inhibited <jats:italic>Helicobacter pylori<\/jats:italic> growth and suppressed the mutagenic effect of metronidazole.<\/jats:p><jats:p><jats:bold>Aim<\/jats:bold> :\u2002To determine the effects of a cyclo\u2010oxygenase (COX)\u20102\u2010specific inhibitor, SC\u2010236, and a non\u2010selective COX inhibitor, indometacin, on the growth, urease activity and antimicrobial susceptibility of <jats:italic>H. pylori<\/jats:italic>.<\/jats:p><jats:p><jats:bold>Methods<\/jats:bold> :\u2002Three <jats:italic>H. pylori<\/jats:italic> reference strains, and 18 clinical isolates were treated with SC\u2010236 or indometacin for 24 and 48\u2003h. Growth, urease activity and susceptibility to clarithromycin and metronidazole of the bacteria were assessed by viable colony counting, spectrophotometry and <jats:italic>E<\/jats:italic>\u2010test respectively.<\/jats:p><jats:p><jats:bold>Results<\/jats:bold> :\u2002SC\u2010236 and indometacin inhibited <jats:italic>H. pylori<\/jats:italic> growth in a dose\u2010dependent manner with the lowest inhibitory concentrations of 0.03 and 0.1\u2003m<jats:sc>m<\/jats:sc>, and the lethal concentrations of 0.09 and 0.3\u2003m<jats:sc>m<\/jats:sc>, respectively. The numbers of CFU\/mL in <jats:italic>Brucella<\/jats:italic> broth containing 0.09\u2003m<jats:sc>m<\/jats:sc> SC\u2010236 were 2 log lower at 24\u2003h, and even 3 log lower at 48\u2003h than that at 0\u2003h (<jats:italic>P<\/jats:italic>\u2003=\u20030.035, compared with the vehicle control). Treatment of 0.3\u2003m<jats:sc>m<\/jats:sc> indometacin reduced the number of CFU\/mL by 1 log at 24\u2003h compared with that at 0\u2003h (<jats:italic>P<\/jats:italic>\u2003=\u20030.037 compared with the vehicle control). <jats:italic>Helicobacter pylori<\/jats:italic> urease activity began to decrease with 0.06\u2003m<jats:sc>m<\/jats:sc> SC\u2010236 at 24\u2003h (<jats:italic>P<\/jats:italic>\u2003=\u20030.016), and 0.3\u2003m<jats:sc>m<\/jats:sc> indometacin at 48\u2003h (<jats:italic>P<\/jats:italic>\u2003=\u20030.025). MICs of metronidazole and clarithromycin against <jats:italic>H. pylori<\/jats:italic> were decreased significantly in the presence of 0.03\u2003m<jats:sc>m<\/jats:sc> SC\u2010236 or 0.1\u2003m<jats:sc>m<\/jats:sc> indometacin (all <jats:italic>P<\/jats:italic>\u2003&lt;\u20030.001).<\/jats:p><jats:p><jats:bold>Conclusion<\/jats:bold> :\u2002Both SC\u2010236 and indometacin suppressed the growth and urease activity of <jats:italic>H. pylori<\/jats:italic> in a dose\u2010dependent manner, and increased its susceptibility to the antibiotics.<\/jats:p>","DOI":"10.1111\/j.1365-2036.2004.02168.x","type":"journal-article","created":{"date-parts":[[2004,12,19]],"date-time":"2004-12-19T09:55:08Z","timestamp":1103450108000},"page":"675-681","source":"Crossref","is-referenced-by-count":14,"title":["Effect of cyclo\u2010oxygenase inhibitors on <scp>Helicobacter pylori<\/scp> susceptibility to metronidazole and clarithromycin"],"prefix":"10.1111","volume":"20","author":[{"given":"Q.","family":"Gu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. 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