{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,5]],"date-time":"2024-07-05T18:34:17Z","timestamp":1720204457473},"reference-count":29,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2006,4,7]],"date-time":"2006-04-07T00:00:00Z","timestamp":1144368000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Aliment Pharmacol Ther"],"published-print":{"date-parts":[[2006,5]]},"abstract":"<jats:sec><jats:title>Summary<\/jats:title><\/jats:sec><jats:sec><jats:title>Background<\/jats:title><jats:p>In coeliac disease, wheat, barley and rye are traditionally excluded in the gluten\u2010free diet. However, few studies have examined the small intestinal immune response to barley and rye.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>To investigate the immunogenicity of barley and rye prolamins (hordein and secalin respectively) in comparison with wheat gliadin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Duodenal biopsies from 22 coeliac patients and 23 disease controls were cultured for 4\u2003h with gliadin, hordein or secalin and compared with culture medium alone. Proinflammatory cytokines, interferon\u2010<jats:italic>\u03b3<\/jats:italic> and interleukin\u20102, were quantified by TaqMan polymerase chain reaction and enzyme\u2010linked immunosorbent assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Hordein caused the greatest increase in interferon\u2010<jats:italic>\u03b3<\/jats:italic> mRNA in coeliac patients (median: 3.3\u2010fold) in comparison with control subjects (median: 0.28\u2010fold, <jats:italic>P<\/jats:italic>\u2003&lt;\u20030.085). Secalin and gliadin induced similar levels of interferon\u2010<jats:italic>\u03b3<\/jats:italic> mRNA with median fold\u2010changes of 3.4 and 2.8, respectively, in coeliac patients in comparison with 1.6\u2010 and 1.1\u2010fold increases in control subjects (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030.294 and <jats:italic>P<\/jats:italic>\u2003&lt;\u20030.105, respectively). The median fold\u2010changes for interleukin\u20102 mRNA did not differ between coeliac patients and controls. Cytokine protein was not upregulated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The findings of this study provide evidence that barley and rye cause immune activation in the mucosa of coeliac patients and support the practice that barley and rye should be excluded from the gluten\u2010free diet.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1365-2036.2006.02876.x","type":"journal-article","created":{"date-parts":[[2006,4,7]],"date-time":"2006-04-07T08:31:27Z","timestamp":1144398687000},"page":"1307-1314","source":"Crossref","is-referenced-by-count":6,"title":["Barley and rye prolamins induce an mRNA interferon\u2010\u03b3 response in coeliac mucosa"],"prefix":"10.1111","volume":"23","author":[{"given":"S. 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