{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T12:52:31Z","timestamp":1790254351855,"version":"4.1.0"},"reference-count":50,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,7,25]],"date-time":"2006-07-25T00:00:00Z","timestamp":1153785600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Aliment Pharmacol Ther"],"published-print":{"date-parts":[[2006,8]]},"abstract":"<jats:sec><jats:title>Summary<\/jats:title><\/jats:sec><jats:sec><jats:title>Background<\/jats:title><jats:p>Defective neutrophil recruitment has been described as a primary pathogenic abnormality in Crohn\u2019s disease. Cantharidin\u2010induced blisters provide a novel investigative tool to assess cellular influx and inflammatory mediator production during acute inflammation and allows the effects of therapy on these parameters to be measured.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aims<\/jats:title><jats:p>To determine whether reduced neutrophil tissue penetration in Crohn's disease relates to impaired production of inflammatory mediators, and whether it can be reversed by granulocyte\u2010colony stimulating factor (G\u2010CSF).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Neutrophil and monocyte\/macrophage populations and inflammatory mediators were measured in cantharidin blisters at 24\u2003h. Neutrophil chemotaxis was assessed <jats:italic>in vitro<\/jats:italic> using blister fluid as the chemoattractant. The effect of s.c. G\u2010CSF on blister phenotype was determined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Significantly fewer neutrophils migrated into blisters in Crohn's patients. The production of neutrophil chemokines, but not other inflammatory mediators, was reduced. This significantly correlated with reduced chemotaxis <jats:italic>in vitro<\/jats:italic>. Differences were unrelated to <jats:italic>caspase\u2010recruitment domain 15<\/jats:italic> genotype. G\u2010CSF significantly increased blister neutrophil concentrations in control subjects and Crohn's patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Reduced neutrophil migration during acute inflammation in Crohn's disease is associated with impaired production of appropriate chemoattractants. G\u2010CSF therapy increases neutrophil tissue migration, which may partially account for its observed therapeutic effect.<\/jats:p><\/jats:sec>","DOI":"10.1111\/j.1365-2036.2006.03016.x","type":"journal-article","created":{"date-parts":[[2006,7,25]],"date-time":"2006-07-25T13:13:45Z","timestamp":1153833225000},"page":"651-660","source":"Crossref","is-referenced-by-count":52,"title":["Impaired neutrophil chemotaxis in Crohn's disease relates to reduced production of chemokines and can be augmented by granulocyte\u2010colony stimulating factor"],"prefix":"10.1111","volume":"24","author":[{"given":"M. W. N.","family":"HARBORD","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. J. B.","family":"MARKS","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"FORBES","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. 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