{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T14:16:58Z","timestamp":1793715418885,"version":"4.6.1"},"reference-count":14,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,2,14]],"date-time":"2005-02-14T00:00:00Z","timestamp":1108339200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Anaesthesia"],"published-print":{"date-parts":[[2005,3]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    The investigation of anaphylactic reactions in the peri\u2010operative period is difficult. Elevation of serum tryptase levels is a good indicator of an anaphylactic event but the ability of subsequent investigations to identify the drug(s) responsible for the reaction is still potentially unreliable. The aim of this study was to examine basophil activation as an investigative tool. We performed flow cytometric analysis of the expression on the cell surface of the basophil activation markers CD63 and CD203c and measured histamine release in 21 patients who were referred with possible peri\u2010operative anaphylaxis. The sensitivity of CD63, CD203c, basophil histamine release and skin prick for the muscle relaxants was found to be 79%, 36%, 36% and 64%, respectively; the specificity was found to be 100%. These results demonstrate the difficulty in investigating the cause of an unexpected clinical event following drug administration, but the higher sensitivity of neo\u2010expression on the cell surface of CD63 suggests that flow cytometric analysis of its neo\u2010expression on basophils\n                    <jats:italic>in vitro<\/jats:italic>\n                    may be a diagnostic aid.\n                  <\/jats:p>","DOI":"10.1111\/j.1365-2044.2004.04086.x","type":"journal-article","created":{"date-parts":[[2005,2,14]],"date-time":"2005-02-14T04:29:36Z","timestamp":1108355376000},"page":"251-256","source":"Crossref","is-referenced-by-count":101,"title":["Flow cytometric investigation of peri\u2010anaesthetic anaphylaxis using CD63 and CD203c"],"prefix":"10.1111","volume":"60","author":[{"given":"P. S.","family":"Sudheer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. E.","family":"Hall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. F.","family":"Read","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. W.","family":"Rowbottom","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. E.","family":"Williams","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,2,14]]},"reference":[{"key":"e_1_2_12_2_2","first-page":"263","article-title":"Anaphylaxis during anaesthesia: current aspects of diagnosis and prevention","volume":"11","author":"Fisher M","year":"1994","journal-title":"European Journal of Anaesthesiology"},{"key":"e_1_2_12_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1081-1206(10)62657-9"},{"key":"e_1_2_12_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.2000.00863.x"},{"key":"e_1_2_12_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(99)70386-6"},{"key":"e_1_2_12_6_2","doi-asserted-by":"publisher","DOI":"10.1006\/clim.2001.5156"},{"key":"e_1_2_12_7_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V97.10.3303"},{"key":"e_1_2_12_8_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.2003.01594.x"},{"key":"e_1_2_12_9_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.2003.00691.x"},{"key":"e_1_2_12_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(91)90094-5"},{"key":"e_1_2_12_11_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.2001.01122.x"},{"key":"e_1_2_12_12_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1398-9995.2001.t01-1-00001.x"},{"key":"e_1_2_12_13_2","volume-title":"Suspected Anaphylactic Reactions Associated with Anaesthesia.","author":"The Association of Anaesthetists of Great Britain and Ireland and British Society for Allergy and Clinical Immunology.","year":"2003"},{"key":"e_1_2_12_14_2","first-page":"45","article-title":"Systemic anaphylaxis induced by intradermal testing","volume":"34","author":"Vincent D","year":"2002","journal-title":"Allergy Immunology"},{"key":"e_1_2_12_15_2","doi-asserted-by":"publisher","DOI":"10.1097\/00000542-199106000-00016"}],"container-title":["Anaesthesia"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2044.2004.04086.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/associationofanaesthetists-publications.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2044.2004.04086.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T22:45:17Z","timestamp":1761691517000},"score":1,"resource":{"primary":{"URL":"https:\/\/associationofanaesthetists-publications.onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2044.2004.04086.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,2,14]]},"references-count":14,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2005,3]]}},"alternative-id":["10.1111\/j.1365-2044.2004.04086.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2044.2004.04086.x","archive":["Portico"],"relation":{},"ISSN":["0003-2409","1365-2044"],"issn-type":[{"value":"0003-2409","type":"print"},{"value":"1365-2044","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,2,14]]}}}