{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T15:46:49Z","timestamp":1762271209992},"reference-count":17,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2008,10,9]],"date-time":"2008-10-09T00:00:00Z","timestamp":1223510400000},"content-version":"vor","delay-in-days":5214,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[1994,7]]},"abstract":"<jats:p><jats:bold>Summary. <\/jats:bold> Previously, a subset of T cells co\u2010expressing the NK cell antigen CD56 has been described. These CD3<jats:sup>+<\/jats:sup>CD56<jats:sup>+<\/jats:sup> cells are rare in peripheral blood collections and have been poorly characterized. We have developed culture conditions which allow for the rapid expansion of CD3<jats:sup>+<\/jats:sup>CD56<jats:sup>+<\/jats:sup> cells. The protocol for cellular expansion includes the addition of interferon\u2010gamma on day O, interleukin\u20101, interleukin\u20102 and a monoclonal antibody against CD3 on day 1 to peripheral blood lymphocytes. Cells of the CD3<jats:sup>+<\/jats:sup>CD56<jats:sup>+<\/jats:sup> phenotype increased up to 6000\u2010fold using this protocol after 16 d in culture. These cells have been characterized by flow cytometry and have been found to express the alpha, beta T cell receptor, co\u2010express the CD5 and CD8 antigens and do not express the CD16 antigen. Morphologically, these cells cannot be distinguished from NK cells. CD3<jats:sup>+<\/jats:sup>CD56<jats:sup>+<\/jats:sup> killer cells lyse a variety of tumour cells with intermediate activity between CD3<jats:sup>\u2212<\/jats:sup>CD56<jats:sup>+<\/jats:sup> NK cells and CD3<jats:sup>+<\/jats:sup>CD56<jats:sup>\u2212<\/jats:sup> T cells.<\/jats:p>","DOI":"10.1111\/j.1365-2141.1994.tb08297.x","type":"journal-article","created":{"date-parts":[[2008,3,12]],"date-time":"2008-03-12T11:59:22Z","timestamp":1205323162000},"page":"453-458","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":91,"title":["Propagation of large numbers of T cells with natural killer cell markers"],"prefix":"10.1111","volume":"87","author":[{"given":"I. G. 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