{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,9,12]],"date-time":"2024-09-12T03:08:35Z","timestamp":1726110515594},"reference-count":21,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,2,21]],"date-time":"2006-02-21T00:00:00Z","timestamp":1140480000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2006,5]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>It has increasingly been acknowledged that only a few leukaemic cells possess the capability to renew themselves and that only these self\u2010renewing leukaemic stem cells are able to initiate relapses. Therefore, these leukaemic stem cells should be the target cells for therapy and for minimal residual disease (MRD) detection. Because of its presence on blasts of 11q23\u2010rearranged high\u2010risk leukaemic patients, neuron\u2010glial antigen 2 (NG2) is thought to be a valuable marker for detecting leukaemic stem cells. Six acute myeloid leukaemia (AML)\/abn(11q23) and three acute lymphoblastic leukaemia (ALL)\/t(4;11) samples were analysed by four\u2010colour flow cytometry for NG2 expression on primitive cell populations. Candidate leukaemic cell populations were defined by the antigen profiles CD34<jats:sup>+<\/jats:sup>CD38<jats:sup>\u2212<\/jats:sup> in AML and CD34<jats:sup>+<\/jats:sup>CD19<jats:sup>\u2212<\/jats:sup>CD117<jats:sup>+<\/jats:sup> in ALL. Surprisingly, in all patients these candidate stem cell populations were shown to lack expression of NG2. Instead, a correlation between the expression of the myeloid differentiation marker CD33 and increasing levels of NG2 on maturing cells could be demonstrated. Similarly, in ALL patients CD34<jats:sup>+<\/jats:sup>CD19<jats:sup>+<\/jats:sup> cells showed a higher expression of <jats:italic>NG2<\/jats:italic> mRNA compared with CD34<jats:sup>+<\/jats:sup>CD19<jats:sup>\u2212<\/jats:sup>. Thus, NG2 appears to be upregulated with differentiation and not to be expressed on primitive disease\u2010maintaining cells. This hampers the clinical use of NG2 as a therapeutic target and as a sensitive marker for MRD detection.<\/jats:p>","DOI":"10.1111\/j.1365-2141.2006.06013.x","type":"journal-article","created":{"date-parts":[[2006,2,21]],"date-time":"2006-02-21T14:57:25Z","timestamp":1140533845000},"page":"337-344","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Lack of expression of the chondroitin sulphate proteoglycan neuron\u2010glial antigen 2 on candidate stem cell populations in paediatric acute myeloid leukaemia\/abn(11q23) and acute lymphoblastic leukaemia\/t(4;11)"],"prefix":"10.1111","volume":"133","author":[{"given":"J.","family":"Neudenberger","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Hotfilder","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Rosemann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Langebrake","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D.","family":"Reinhardt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Pieters","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Schrauder","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Schrappe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"R\u00f6ttgers","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Harbott","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Vormoor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,3,31]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1999.01746.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"crossref","first-page":"1134","DOI":"10.1182\/blood.V87.3.1134.bloodjournal8731134","article-title":"Human 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