{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T01:12:23Z","timestamp":1791162743611,"version":"4.1.0"},"reference-count":50,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,3,31]],"date-time":"2006-03-31T00:00:00Z","timestamp":1143763200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2006,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>To examine the usage and mutational status of VH genes in hairy cell leukaemia (HCL), we analysed 24 immunoglobulin heavy chain (IgH) sequences expressed in 23 patients. None had premature stop codons. VH3\u201023 was the most common gene and a VH6 gene was observed for the first time in HCL. Although the mean mutation frequency was 6\u00b71%, slightly higher than in previous HCL series, four patients had 99\u00b76\u2013100% homology to germline sequences, three of whom had high tumour burdens and poor outcomes. Despite the high mutation frequency, only two of 24 rearrangements had clear statistical evidence of antigen\u2010dependent somatic mutation. Our results increase the database of reported functional HCL rearrangements to 94 in 92 patients. Overall, both gene usage and mutation frequency are very similar to mucosa\u2010associated lymphoid tissue\u2010type marginal zone lymphoma. The data are consistent with HCL originating from post\u2010germinal centre marginal zone B cells, although the heterogeneity observed suggests that HCL may originate differently in some patients, and this could have implications for prognosis and treatment.<\/jats:p>","DOI":"10.1111\/j.1365-2141.2006.06066.x","type":"journal-article","created":{"date-parts":[[2006,3,31]],"date-time":"2006-03-31T14:50:58Z","timestamp":1143816658000},"page":"504-512","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Somatic hypermutation and VH gene usage in hairy cell leukaemia"],"prefix":"10.1111","volume":"133","author":[{"given":"Evgeny","family":"Arons","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joel","family":"Sunshine","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tara","family":"Suntum","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert J.","family":"Kreitman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,4,27]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V65.3.620.620"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1521-6926(02)00087-7"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119433"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-85226-8_17"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(94)90175-9"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0694-162"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V94.6.1840"},{"key":"e_1_2_6_9_1","doi-asserted-by":"crossref","first-page":"3953","DOI":"10.1182\/blood.V88.10.3953.bloodjournal88103953","article-title":"High frequency of somatic mutations in the VH genes expressed in prolymphocytic leukemia","volume":"88","author":"Davi F.","year":"1996","journal-title":"Blood"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2403202"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-4141(199810)28:10<3384::AID-IMMU3384>3.0.CO;2-T"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0046-8177(98)80007-5"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/83.3.289"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V98.4.1174"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1977.tb00595.x"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-89-5-677"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V94.6.1848"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1988.tb03309.x"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1995.tb55820.x"},{"key":"e_1_2_6_20_1","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1182\/blood.V59.1.52.52","article-title":"Cell markers in hairy cell leukemia studied in cells from 51 patients","volume":"59","author":"Jansen J.","year":"1982","journal-title":"Blood"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/281605a0"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199911113412007"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.9.5122"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1998.00653.x"},{"issue":"1","key":"e_1_2_6_25_1","first-page":"57","article-title":"The immunophenotype of hairy cell leukemia (HCL). 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