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Abnormal methylation of <jats:italic>DBC1<\/jats:italic> was observed in all ALL cell lines and in 17% of ALL patients. Moreover, <jats:italic>DBC1<\/jats:italic> methylation was associated with decreased <jats:italic>DBC1<\/jats:italic> expression, while treatment of ALL cells with 5\u2010Aza\u20102\u2032\u2010deoxycytidine resulted in demethylation of the promoter and upregulation of <jats:italic>DBC1<\/jats:italic> expression. Fluorescence <jats:italic>in situ<\/jats:italic> hybridisation identified the deletion of one allele of <jats:italic>DBC1<\/jats:italic> in some ALL cell lines, which indicated that the lack of <jats:italic>DBC1<\/jats:italic> expression was due to deletion of one allele and methylation of the other. In conclusion, these results demonstrate, for the first time, that the expression of <jats:italic>DBC1<\/jats:italic> is downregulated in a percentage of patients with ALL due to the hypermethylation of its promoter and\/or gene deletion.<\/jats:p>","DOI":"10.1111\/j.1365-2141.2006.06131.x","type":"journal-article","created":{"date-parts":[[2006,5,23]],"date-time":"2006-05-23T13:43:07Z","timestamp":1148391787000},"page":"137-144","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Downregulation of <i>DBC1<\/i> expression in acute lymphoblastic leukaemia is mediated by aberrant methylation of its promoter"],"prefix":"10.1111","volume":"134","author":[{"given":"Edurne","family":"San 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