{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T13:54:26Z","timestamp":1761918866460},"reference-count":46,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,9,22]],"date-time":"2006-09-22T00:00:00Z","timestamp":1158883200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2006,11]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>In this pilot study, T\u2010cell receptor B\u2010variable (TCR\u2010BV) gene usage in CD4 and CD8 T cells was assessed, by real\u2010time polymerase chain reaction, as well as complementarity\u2010determining region 3 (CDR3)\u2010length polymorphism, before and after therapy in five patients with B\u2010cell chronic lymphocytic leukaemia who received alemtuzumab (anti\u2010CD52 monoclonal antibody) as first\u2010line therapy. A decline in expression of most BV family genes in both CD4 and CD8 T cells was observed after alemtuzumab treatment, which was followed by a gradual increase in most BV families during long\u2010term follow\u2010up. After treatment, CDR3\u2010length polymorphism showed an even more restricted pattern in CD4 T cells compared with pretreatment, with a shift towards a monoclonal\/oligoclonal pattern. The clonally restricted pattern was significantly reduced in CD4 (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030\u00b701) but not in CD8 T cells. This was followed by a gradual increase in the number of peaks within the CDR3 region of the different TCR\u2010BV families, i.e. a polyclonal repertoire, during long\u2010term follow\u2010up. A restricted CDR3 pattern became even more restricted after treatment, but normalised during unmaintained follow\u2010up. These results indicate that perturbations in the T\u2010cell alterations following alemtuzumab are complex and include not only changes in CD4\/CD8 T\u2010cell numbers but also a highly restricted T\u2010cell repertoire especially in CD4 T cells.<\/jats:p>","DOI":"10.1111\/j.1365-2141.2006.06324.x","type":"journal-article","created":{"date-parts":[[2006,9,22]],"date-time":"2006-09-22T13:15:16Z","timestamp":1158930916000},"page":"475-485","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Reconstitution of the T\u2010cell repertoire following treatment with alemtuzumab (anti\u2010CD52 monoclonal antibody) in patients with B\u2010cell chronic lymphocytic leukaemia"],"prefix":"10.1111","volume":"135","author":[{"given":"Mohammad Reza","family":"Rezvany","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahmood Jeddi","family":"Tehrani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claes","family":"Karlsson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeanette","family":"Lundin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hodjattallah","family":"Rabbani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anders","family":"\u00d6sterborg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H\u00e5kan","family":"Mellstedt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,10,16]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(00)00136-8"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2003.04679.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1385\/MO:19:4:277"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90021-2"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/334395a0"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-200004150-00022"},{"key":"e_1_2_6_8_1","doi-asserted-by":"crossref","first-page":"4281","DOI":"10.4049\/jimmunol.153.9.4281","article-title":"T\u2010cell repertoire in patients with B chronic lymphocytic leukemia. 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