{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,2]],"date-time":"2026-03-02T11:34:41Z","timestamp":1772451281742,"version":"3.50.1"},"reference-count":24,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,12,8]],"date-time":"2006-12-08T00:00:00Z","timestamp":1165536000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2007,1]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Growth factor\u2010independent 1B (<jats:italic>GFI1B<\/jats:italic>) is a transcription factor essential for the development and differentiation of erythroid and megakaryocytic lineages. We evaluated the <jats:italic>GFI1B<\/jats:italic> expression in erythroleukaemia and megakaryocytic leukaemia, as well as in patients with other subtypes of acute myeloid leukaemia (AML), acute lymphoblastic leukaemia (ALL), chronic myeloid leukaemia (CML), myelodysplastic syndrome (MDS), severe aplastic anaemia (SAA), myelofibrosis with myeloid metaplasia (MMM) and in healthy volunteers. GFI1B expression was increased at least threefold in patients with erythroleukaemia (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030\u00b701 compared with controls) and megakaryocytic leukaemia (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030\u00b705) as well as in their corresponding leukaemic cell lines HEL, K562, CMK and M\u201007e. Patients with undifferentiated or monocytic AML, ALL, MMM, MDS and CML had no significantly altered <jats:italic>GFI1B<\/jats:italic> expression, whereas <jats:italic>GFI1B<\/jats:italic> expression was decreased 10\u2010fold in patients with SAA (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030\u00b70001 compared with controls). Silencing <jats:italic>GFI1B<\/jats:italic> by transfection with small interfering RNA (siRNA) markedly reduced the proliferation rate in the leukaemic cell lines HEL, K562 and NB4 (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030\u00b701). Concomitantly, we observed a two\u2010 to threefold increase in the apoptosis rate in these cells after transfection with siRNA towards <jats:italic>GFI1B<\/jats:italic>. Our data indicate that <jats:italic>GFI1B<\/jats:italic> plays a major role in AML\u2010M6 and AML\u2010M7 and qualifies as a target for anti\u2010leukaemic strategies in these malignancies.<\/jats:p>","DOI":"10.1111\/j.1365-2141.2006.06407.x","type":"journal-article","created":{"date-parts":[[2006,12,8]],"date-time":"2006-12-08T12:32:32Z","timestamp":1165581152000},"page":"212-219","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Growth factor\u2010independent 1B gene (<i>GFI1B<\/i>) is overexpressed in erythropoietic and megakaryocytic malignancies and increases their proliferation rate"],"prefix":"10.1111","volume":"136","author":[{"given":"Ahmet H.","family":"Elmaagacli","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Koldehoff","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johannes L.","family":"Zakrzewski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nina K.","family":"Steckel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hellmut","family":"Ottinger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dietrich W.","family":"Beelen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,12,8]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1031694100"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/35078107"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2001.02858.x"},{"key":"e_1_2_6_5_1","first-page":"326","article-title":"WT1 and BCR\u2010ABL specific small interfering RNA have additive effects in the induction of apoptosis in leukemic cells","volume":"90","author":"Elmaagacli A.H.","year":"2005","journal-title":"Haematologica"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2003-11-4068"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.13.3.1759"},{"key":"e_1_2_6_8_1","doi-asserted-by":"crossref","unstructured":"Grimes H.L. 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