{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T20:55:04Z","timestamp":1791752104231,"version":"4.3.4"},"reference-count":37,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,12,13]],"date-time":"2006-12-13T00:00:00Z","timestamp":1165968000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2007,2]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Thromboembolic complications have been documented in thalassaemia patients. The aggregability of abnormal red blood cells and the high level of membrane\u2010derived microparticles (MPs) stemming from blood cells are thought to be responsible for the associated thrombotic risk. We investigated the number of MPs, their cellular origin and their procoagulant properties in <jats:italic>\u03b2<\/jats:italic>\u2010thalassaemia. Fresh whole blood was simultaneously stained for annexin V, cellular antigens and the known density beads. The procoagulant properties of these phosphatidylserine (PS)\u2010bearing MPs were also measured by assessing the platelet factor\u20103\u2010like activity in the blood. Flow cytometric results showed that splenectomised <jats:italic>\u03b2<\/jats:italic>\u2010thalassaemia\/HbE patients had significantly higher levels of PS\u2010bearing MPs than non\u2010splenectomised <jats:italic>\u03b2<\/jats:italic>\u2010thalassaemia\/HbE patients and normal individuals (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030\u00b70001). There was a good correlation between PS\u2010bearing MPs and PS\u2010bearing platelets, reflecting the existence of chronic platelet activation in <jats:italic>\u03b2<\/jats:italic>\u2010thalassaemia\/HbE patients (<jats:italic>r<\/jats:italic><jats:sub>s<\/jats:sub>\u2003=\u20030\u00b7511, <jats:italic>P<\/jats:italic>\u2003&lt;\u20030\u00b7001). The cellular origin of PS\u2010bearing MPs showed mostly activated\u2010platelet origin with adhesion (CD41a\/CD62P\/CD36). Moreover, the platelet procoagulant activity was higher in splenectomised <jats:italic>\u03b2<\/jats:italic>\u2010thalassaemia\/HbE patients when compared with non\u2010splenectomised (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030\u00b705) and normal individuals (<jats:italic>P<\/jats:italic>\u2003&lt;\u20030\u00b701), and the amount correlated with PS\u2010bearing MPs (<jats:italic>r<\/jats:italic><jats:sub>s<\/jats:sub>\u2003=\u20030\u00b7560, <jats:italic>P<\/jats:italic>\u2003&lt;\u20030\u00b7001). These findings suggest that MPs originate from activated platelets with a potential to aggravate thrombotic events when the numbers are excessive, as is commonly seen in splenectomised <jats:italic>\u03b2<\/jats:italic>\u2010thalassaemia\/HbE patients.<\/jats:p>","DOI":"10.1111\/j.1365-2141.2006.06449.x","type":"journal-article","created":{"date-parts":[[2006,12,14]],"date-time":"2006-12-14T03:53:15Z","timestamp":1166068395000},"page":"462-471","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":70,"title":["Activated platelet\u2010derived microparticles in 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