{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T02:59:22Z","timestamp":1785898762560,"version":"3.56.0"},"reference-count":21,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2010,1,11]],"date-time":"2010-01-11T00:00:00Z","timestamp":1263168000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2010,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Metaphase (M\u2010) and array (A\u2010) Comparative Genomic Hybridization (CGH) were used to investigate 40 cases of T\u2010 and 32 of B\u2010cell acute lymphoblastic leukaemia (ALL) with normal\/failed cytogenetics. M\u2010CGH was performed in all cases and A\u2013CGH in 10\/12 T\u2010ALL cases with uncertain\/normal M\u2010CGH results. M\u2010CGH was abnormal in 38\/72 cases, with a total of 110 imbalances (60 gains, 50 losses). 25\/40 patients with T\u2010ALL (62\u00b75%) showed 77 imbalances, with at least 1 genomic imbalance and a mean of 3 aberrations\/patient (range 1\u201312). 13\/32 patients with B\u2010ALL (40\u00b76%) presented 34 imbalances, with a mean of 2\u00b76 imbalances (range 1\u20138). A\u2010CGH detected 4 more T\u2010ALL cases with genomic imbalances. A\u2010CGH identified <jats:italic>NF1<\/jats:italic>\/17q11\u00b72 deletion and interphase fluorescence <jats:italic>in situ<\/jats:italic> hybridization provided a 10\u00b78% estimated overall incidence of <jats:italic>NF1<\/jats:italic>\/17q11\u00b72 deletion in T\u2010ALL. In all but one case (6\/7) with <jats:italic>NF1<\/jats:italic> deletion, denaturing high\u2010performance liquid chromatography and direct sequencing detected <jats:italic>NOTCH1<\/jats:italic> gene mutations. Three or more imbalances in CGH\u2010positive cases were significantly associated with resistance to treatment and death during or after induction therapy. We suggest that the work\u2010up for ALL at diagnosis should include CGH investigations, particularly when cytogenetics is uninformative, because they may provide potentially valuable information with prognostic and therapeutic implications.<\/jats:p>","DOI":"10.1111\/j.1365-2141.2009.08056.x","type":"journal-article","created":{"date-parts":[[2010,1,11]],"date-time":"2010-01-11T23:01:53Z","timestamp":1263250913000},"page":"70-78","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Rescue of genomic information in adult acute lymphoblastic leukaemia (ALL) with normal\/failed cytogenetics: a GIMEMA centralized biological 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