{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,28]],"date-time":"2023-11-28T00:31:13Z","timestamp":1701131473549},"reference-count":10,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2010,1,11]],"date-time":"2010-01-11T00:00:00Z","timestamp":1263168000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2010,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Isolation and sequencing of the translocation breakpoint in chronic myeloid leukaemia\u2010(CML) and acute promyelocytic leukaemia (APML) may help to elucidate the mechanism of translocation and provide a molecular marker for monitoring of minimal residual disease. Amplification across the translocation breakpoint was performed in samples from 91 patients with CML and 15 patients with APML using single\u2010tube multiplex polymerase chain reaction (PCR) involving 308 primers for CML and 40 primers for APML. Nonspecific amplification was removed by a modification of PCR, termed sequential hybrid primer PCR (SHP\u2010PCR), which involved two sequential rounds of PCR, each of which used a low concentration of a specially designed hybrid primer. The resultant amplified material was sequenced. The method as finally developed was simple quick and robust. The translocation breakpoint was successfully isolated and sequenced in all 106 samples. The strategy of highly multiplexed PCR followed by SHP\u2010PCR is thus an effective method for isolating the translocation breakpoint in CML and APML. It may also be applicable to other haematological disorders characterised by translocation, deletion or inversion.<\/jats:p>","DOI":"10.1111\/j.1365-2141.2009.08071.x","type":"journal-article","created":{"date-parts":[[2010,1,12]],"date-time":"2010-01-12T04:02:03Z","timestamp":1263268923000},"page":"231-236","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Rapid isolation of translocation breakpoints in chronic myeloid and acute promyelocytic leukaemia"],"prefix":"10.1111","volume":"149","author":[{"given":"Paul A.","family":"Bartley","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael H.","family":"Martin\u2010Harris","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bradley J.","family":"Budgen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David M.","family":"Ross","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander A.","family":"Morley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,3,22]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.16.3235"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.2144\/96213pf02"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-09-110189"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.20568"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/27.6.1558"},{"key":"e_1_2_8_7_1","unstructured":"Morley A.A.(2008)A method for DNA breakpoint analysis. World Intellectual Property Organisation International Publication Number WO 2008\/1434841."},{"key":"e_1_2_8_8_1","unstructured":"Morley A.A.(2009)A method of DNA amplification. World Intellectual Property Organisation International Publication Number WO 2009\/052547."},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/23.6.1087"},{"key":"e_1_2_8_10_1","first-page":"4146","article-title":"Long\u2010template DNA polymerase chain reaction for the detection of the bcr\/abl translocation in patients with chronic myelogenous leukemia","volume":"5","author":"Waller C.F.","year":"1999","journal-title":"Clinical Cancer Research"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1995.tb03392.x"}],"container-title":["British Journal of Haematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2141.2009.08071.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2141.2009.08071.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T02:56:35Z","timestamp":1697424995000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2141.2009.08071.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,3,22]]},"references-count":10,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2010,4]]}},"alternative-id":["10.1111\/j.1365-2141.2009.08071.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2141.2009.08071.x","archive":["Portico"],"relation":{},"ISSN":["0007-1048","1365-2141"],"issn-type":[{"value":"0007-1048","type":"print"},{"value":"1365-2141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,3,22]]},"assertion":[{"value":"2010-03-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}