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Based on these findings, we hypothesized that IgM anti\u2010\u03b2<jats:sub>2<\/jats:sub>M mAbs might have stronger apoptotic effects because of their pentameric structure. Our results showed that, compared with IgG mAbs, IgM anti\u2010\u03b2<jats:sub>2<\/jats:sub>M mAbs exhibited stronger tumouricidal activity <jats:italic>in vitro<\/jats:italic> against different tumour cells, including myeloma, mantle cell lymphoma, and prostate cancer, and <jats:italic>in vivo<\/jats:italic> in a human\u2010like xenografted myeloma mouse model without damaging normal tissues. IgM mAb\u2010induced apoptosis is dependent on the pentameric structure of the mAbs. Disrupting pentameric IgM into monomeric IgM significantly reduced their ability to induce cell apoptosis. Monomeric IgM mAbs were less efficient at recruiting MHC class I molecules into and exclusion of cytokine receptors from lipid rafts, and at activating the intrinsic apoptosis cascade. Thus, we developed and validated the efficacy of anti\u2010\u03b2<jats:sub>2<\/jats:sub>M IgM mAbs that may be utilized in the clinical setting and showed that IgM anti\u2010\u03b2<jats:sub>2<\/jats:sub>M mAbs may be more potent than IgG mAbs at inducing tumour apoptosis.<\/jats:p>","DOI":"10.1111\/j.1365-2141.2011.08714.x","type":"journal-article","created":{"date-parts":[[2011,5,10]],"date-time":"2011-05-10T10:37:04Z","timestamp":1305023824000},"page":"111-121","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Targeting cell surface \u03b2<sub>2<\/sub>\u2010microglobulin by pentameric IgM 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