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We analysed the methylation status of the three members of the <jats:italic>MIR9<\/jats:italic> family (<jats:italic>MIR9\u20101, MIR9\u20102 and MIR9\u20103<\/jats:italic>) in a uniformly treated cohort of 200 newly diagnosed ALLs. <jats:italic>MIR9<\/jats:italic> was methylated in 54% of the patients and was associated with downregulation of <jats:italic>MIR9<\/jats:italic> (<jats:italic>P\u2003<\/jats:italic>&lt;<jats:italic>\u2003<\/jats:italic>0\u00b701). Hypermethylation of <jats:italic>MIR9<\/jats:italic> was an independent prognostic factor for disease\u2010free survival, overall survival and event\u2010free survival in a multivariate analysis (<jats:italic>P\u2003<\/jats:italic>&lt;<jats:italic>\u2003<\/jats:italic>0\u00b701). Epigenetic downregulation of <jats:italic>MIR9<\/jats:italic> induced upregulation of its targets, <jats:italic>FGFR1<\/jats:italic> and <jats:italic>CDK6<\/jats:italic>, while treatment of ALL cells with FGFR1 (PD\u2010173074) and CDK6 (PD\u20100332991) inhibitors induced a decrease in cell proliferation and an increase in apoptosis of ALL cells. Our results indicate that the <jats:italic>MIR9<\/jats:italic> family is involved in the pathogenesis and clinical behaviour of ALL and provide the basis for new therapeutic strategies in the treatment of ALL, targeting the epigenetic regulation of miRNAs and\/or the FGFR1 or CDK6\u2010RB pathway directly.<\/jats:p>","DOI":"10.1111\/j.1365-2141.2011.08812.x","type":"journal-article","created":{"date-parts":[[2011,8,2]],"date-time":"2011-08-02T19:55:11Z","timestamp":1312314911000},"page":"73-83","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":51,"title":["Deregulation of <i>FGFR1<\/i> and <i>CDK6<\/i> oncogenic pathways in acute lymphoblastic leukaemia harbouring epigenetic modifications of the <i>MIR9<\/i> 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