{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T01:30:00Z","timestamp":1774315800245,"version":"3.50.1"},"reference-count":18,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,10,21]],"date-time":"2011-10-21T00:00:00Z","timestamp":1319155200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Gene expression profiling (GEP) of primary leukaemic cells (PLC) from 157 paediatric B\u2010lineage acute lymphoblastic leukaemia (ALL) patients, including a direct comparison of matched pair initial diagnosis <jats:italic>versus<\/jats:italic> first relapse leukaemic specimens, provided previously unknown evidence that relapse clones are characterized by significantly higher expression levels of a <jats:italic>CD22<\/jats:italic> exon 12 deletion (CD22\u0394E12)\u2010associated signature transcriptome than the PLC from newly diagnosed patients. In agreement with and validating these GEP results, reverse transcription polymerase chain reaction and Western blot analysis of PLC from 19 of 19 paediatric ALL patients in first bone marrow relapse occurring within 12\u2003months of the completion of primary therapy confirmed them to be CD22\u0394E12<jats:sup>+<\/jats:sup>. Likewise, PLC in diagnostic initial bone marrow specimens from seven of seven therapy\u2010refractory newly diagnosed paediatric B\u2010lineage ALL patients with &lt;7\u2003months event\u2010free survival (EFS), including four patients with induction failures and three patients with early relapses, were CD22\u0394E12<jats:sup>+<\/jats:sup>, whereas PLC from only one of five newly diagnosed paediatric B\u2010lineage ALL patients with &gt;18\u2003months EFS was CD22\u0394E12<jats:sup>+<\/jats:sup>. CD22\u0394E12<jats:sup>+<\/jats:sup> could be detected in PLC of therapy\u2010refractory patients both at the time of initial diagnosis as well as at the time of documented treatment failure. Our study implicates the CD22\u0394E12 genetic defect in the aggressive biology of relapsed or therapy\u2010refractory paediatric B\u2010lineage ALL.<\/jats:p>","DOI":"10.1111\/j.1365-2141.2011.08901.x","type":"journal-article","created":{"date-parts":[[2011,10,21]],"date-time":"2011-10-21T16:06:07Z","timestamp":1319213167000},"page":"89-98","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["CD22 Exon 12 deletion is a characteristic genetic defect of therapy\u2010refractory clones in paediatric acute lymphoblastic leukaemia"],"prefix":"10.1111","volume":"156","author":[{"given":"Hong","family":"Ma","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanjive","family":"Qazi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zahide","family":"Ozer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Gaynon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gregory H.","family":"Reaman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fatih M.","family":"Uckun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,10,21]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(08)70229-8"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-bioeng-061008-124920"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/jmg.2004.029538"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-02-002824"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.4161\/rna.7.4.12153"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80554-3"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2005.04.5856"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1525"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2007.10.7508"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.274.5288.798"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0506-500"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2005.05572.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2009.286"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2776(05)88001-0"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1007896107"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0909086107"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1586\/era.10.203"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-1910"}],"container-title":["British Journal of Haematology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2141.2011.08901.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2141.2011.08901.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T02:37:42Z","timestamp":1697423862000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2141.2011.08901.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,10,21]]},"references-count":18,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2012,1]]}},"alternative-id":["10.1111\/j.1365-2141.2011.08901.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2141.2011.08901.x","archive":["Portico"],"relation":{},"ISSN":["0007-1048","1365-2141"],"issn-type":[{"value":"0007-1048","type":"print"},{"value":"1365-2141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,10,21]]},"assertion":[{"value":"2011-10-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}