{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T08:08:20Z","timestamp":1791101300941,"version":"4.1.0"},"reference-count":43,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2012,2,24]],"date-time":"2012-02-24T00:00:00Z","timestamp":1330041600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2012,5]]},"abstract":"Summary<\/jats:title>Tissue Factor (TF<\/jats:styled-content>) initiates thrombin generation, and whole blood TF<\/jats:styled-content> (WBTF<\/jats:styled-content>) is elevated in sickle cell disease (SCD<\/jats:styled-content>). We sought to identify the presence of TF<\/jats:styled-content>\u2010positive monocytes in SCD<\/jats:styled-content> and their relationship with the other coagulation markers including WBTF<\/jats:styled-content>, microparticle\u2010associated TF<\/jats:styled-content>, thrombin\u2010antithrombin (TAT<\/jats:styled-content>) complexes and D<\/jats:styled-content>\u2010dimer. Whether major SCD<\/jats:styled-content>\u2010related pathobiological processes, including haemolysis, inflammation and endothelial activation, contribute to the coagulation abnormalities was also studied. The cohort comprised children with SCD<\/jats:styled-content> (18 H<\/jats:styled-content>bSS<\/jats:styled-content>, 12 H<\/jats:styled-content>bSC<\/jats:styled-content>, mean age 3\u00b76\u00a0years). We demonstrated elevated levels of TF<\/jats:styled-content>\u2010positive monocytes in H<\/jats:styled-content>bSS<\/jats:styled-content>, which correlated with WBTF<\/jats:styled-content>, TAT<\/jats:styled-content> and D<\/jats:styled-content>\u2010dimer (P<\/jats:italic>\u00a0=\u00a00\u00b702 to P<\/jats:italic>\u00a0=\u00a00\u00b70003). While TF<\/jats:styled-content>\u2010positive monocytes, WBTF<\/jats:styled-content>, TAT<\/jats:styled-content> and D<\/jats:styled-content>\u2010dimer correlated with several biomarkers of haemolysis, inflammation and endothelial activation in univariate analyses, in multiple regression models the haemolytic markers (reticulocytes and lactate dehydrogenase) contributed exclusively to the association with all four coagulant markers evaluated. The demonstration that haemolysis is the predominant operative pathology in the associated perturbations of coagulation in H<\/jats:styled-content>bSS<\/jats:styled-content> at a young age provides additional evidence for the early use of therapeutic agents, such as hydroxycarbamide to reduce the haemolytic component of this disease.<\/jats:p>","DOI":"10.1111\/j.1365-2141.2012.09065.x","type":"journal-article","created":{"date-parts":[[2012,2,24]],"date-time":"2012-02-24T05:57:32Z","timestamp":1330063052000},"page":"370-380","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":63,"title":["Tissue factor\u2010positive monocytes in children with sickle cell disease: correlation with biomarkers of haemolysis"],"prefix":"10.1111","volume":"157","author":[{"given":"B. N. Yamaja","family":"Setty","sequence":"first","affiliation":[{"name":"Division of Research Haematology Department of Pediatrics Marian Anderson Comprehensive Sickle Cell Anaemia Care and Research Centre Jefferson Medical College Thomas Jefferson University Philadelphia PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nigel S.","family":"Key","sequence":"additional","affiliation":[{"name":"Department of Medicine University of North Carolina at Chapel Hill Chapel Hill NC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. Koneti","family":"Rao","sequence":"additional","affiliation":[{"name":"Sol Sherry Thrombosis Research Centre, Temple University School of Medicine Philadelphia PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suhita","family":"Gayen\u2010Betal","sequence":"additional","affiliation":[{"name":"Division of Research Haematology Department of Pediatrics Marian Anderson Comprehensive Sickle Cell Anaemia Care and Research Centre Jefferson Medical College Thomas Jefferson University Philadelphia PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suba","family":"Krishnan","sequence":"additional","affiliation":[{"name":"Division of Research Haematology Department of Pediatrics Marian Anderson Comprehensive Sickle Cell Anaemia Care and Research Centre Jefferson Medical College Thomas Jefferson University Philadelphia PA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlton 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