{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T02:09:16Z","timestamp":1791425356059,"version":"4.3.3"},"reference-count":26,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,5,25]],"date-time":"2012-05-25T00:00:00Z","timestamp":1337904000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"GOELAMS"},{"name":"DRCI"},{"name":"CHU de Grenoble"},{"name":"French MCL network"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Br J Haematol"],"published-print":{"date-parts":[[2012,7]]},"abstract":"Summary<\/jats:title>\n \n Molecular minimal residual disease (\n MRD<\/jats:styled-content>\n ) analysis is fast emerging as an essential clinical decision\u2010making tool for the treatment and follow\u2010up of mature B cell malignancies. Current Euro\n MRD<\/jats:styled-content>\n consensus\n \n IGH<\/jats:styled-content>\n <\/jats:italic>\n real\u2010time quantitative polymerase chain reaction\n RQ<\/jats:styled-content>\n \u2010\n PCR<\/jats:styled-content>\n assays rely on flow cytometric assessment of diagnostic tumour burdens to construct \u2018normalized\u2019, patient\u2010specific, diagnostic\n DNA<\/jats:styled-content>\n \u2010based\n MRD<\/jats:styled-content>\n quantification standards. Here, we propose a new \u2018hybrid\u2019 assay that relies on plasmid\u2010based quantification of patient\u2010specific\n \n IGH VDJ<\/jats:styled-content>\n <\/jats:italic>\n targets by consensus\n \n IGH<\/jats:styled-content>\n <\/jats:italic>\n real time (\n RQ<\/jats:styled-content>\n )\u2010\n PCR<\/jats:styled-content>\n , combined with Euro\n MRD<\/jats:styled-content>\n guidelines, for\n MRD<\/jats:styled-content>\n monitoring in lymphoid malignancies. This assay was evaluated for\n MRD<\/jats:styled-content>\n assessment in a total of 273 samples from 29 mantle cell lymphoma (\n MCL<\/jats:styled-content>\n ) patients treated within a Groupe Ouest Est d'Etude des Leuc\u00e9mies et Autres Maladies du Sang (GOELAMS) Phase\n II<\/jats:styled-content>\n trial and was feasible, reliable and consistently comparable to gold\u2010standard\n MRD<\/jats:styled-content>\n techniques (99% concordance across all samples including 32 samples within the quantitative range) when analysed in parallel (117 samples). Integrating clinical prognostic parameters and\n MRD<\/jats:styled-content>\n status in peripheral blood at the post\u2010induction stage was predictive of progression\u2010free survival (\n P<\/jats:italic>\n \u00a0=\u00a00\u00b7034) thus demonstrating the clinical utility of the approach. Plasmid\u2010based standards for the quantification of\n \n IGH VDJ<\/jats:styled-content>\n <\/jats:italic>\n targets are therefore confirmed to offer new opportunities for further standardization and clinical evaluation of\n MRD<\/jats:styled-content>\n \u2010guided management of patients with mature B cell malignancies.\n <\/jats:p>","DOI":"10.1111\/j.1365-2141.2012.09161.x","type":"journal-article","created":{"date-parts":[[2012,5,25]],"date-time":"2012-05-25T03:35:30Z","timestamp":1337916930000},"page":"186-197","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Cloned\n \n IGH VDJ<\/scp>\n <\/i>\n targets as tools for personalized minimal residual disease monitoring in mature lymphoid malignancies; a feasibility study in mantle cell lymphoma by the Groupe Ouest Est d'Etude des Leuc\u00e9mies et Autres Maladies du Sang"],"prefix":"10.1111","volume":"158","author":[{"given":"Estelle","family":"Gimenez","sequence":"first","affiliation":[{"name":"INSERM U823, Institut Albert Bonniot Grenoble France"},{"name":"Universit\u00e9 Joseph Fourier\u2010Grenoble I Institut Albert Bonniot Grenoble France"},{"name":"Plateforme de g\u00e9n\u00e9tique mol\u00e9culaire des cancers, P\u00f4le de Biologie CHU de Grenoble Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martine","family":"Chauvet","sequence":"additional","affiliation":[{"name":"INSERM U823, Institut Albert Bonniot Grenoble France"},{"name":"Universit\u00e9 Joseph Fourier\u2010Grenoble I Institut Albert Bonniot Grenoble France"},{"name":"Plateforme de g\u00e9n\u00e9tique mol\u00e9culaire des cancers, P\u00f4le de Biologie CHU de Grenoble Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laetitia","family":"Rabin","sequence":"additional","affiliation":[{"name":"Plateforme de g\u00e9n\u00e9tique mol\u00e9culaire des cancers, P\u00f4le de Biologie CHU de Grenoble Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Puteaud","sequence":"additional","affiliation":[{"name":"Plateforme de g\u00e9n\u00e9tique mol\u00e9culaire des cancers, P\u00f4le de Biologie CHU de Grenoble Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel","family":"Duley","sequence":"additional","affiliation":[{"name":"INSERM U823, Institut Albert Bonniot Grenoble France"},{"name":"Universit\u00e9 Joseph Fourier\u2010Grenoble I Institut Albert Bonniot Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sieme","family":"Hamaidia","sequence":"additional","affiliation":[{"name":"INSERM U823, Institut Albert Bonniot Grenoble France"},{"name":"Universit\u00e9 Joseph Fourier\u2010Grenoble I Institut Albert Bonniot Grenoble France"},{"name":"P\u00f4le recherche CHU de Grenoble Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana","family":"Bruder","sequence":"additional","affiliation":[{"name":"INSERM U823, Institut Albert Bonniot Grenoble France"},{"name":"Universit\u00e9 Joseph Fourier\u2010Grenoble I Institut Albert Bonniot Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9rie","family":"Rolland\u2010Neyret","sequence":"additional","affiliation":[{"name":"P\u00f4le recherche CHU de Grenoble Grenoble France"},{"name":"P\u00f4le de Canc\u00e9rologie et d'H\u00e9matologie CHU de Grenoble Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven","family":"Le Gouill","sequence":"additional","affiliation":[{"name":"Service d'H\u00e9matologie clinique CHU de Nantes Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Tournilhac","sequence":"additional","affiliation":[{"name":"H\u00e9matologie H\u00f4tel Dieu CHU de Clermont Ferrand, Boulevard Leon Malfreyt Clermont Ferrand France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Voog","sequence":"additional","affiliation":[{"name":"Service d'H\u00e9matologie Clinique Victor Hugo, 1 rue Victor Hugo Le Mans France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herv\u00e9","family":"Maisonneuve","sequence":"additional","affiliation":[{"name":"Service d'H\u00e9matologie La Roche sur Yon France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie C.","family":"Jacob","sequence":"additional","affiliation":[{"name":"INSERM U823, Institut Albert Bonniot Grenoble France"},{"name":"Universit\u00e9 Joseph Fourier\u2010Grenoble I Institut Albert Bonniot Grenoble France"},{"name":"Laboratoire d'immunologie cellulaire CHU de Grenoble Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Leroux","sequence":"additional","affiliation":[{"name":"INSERM U823, Institut Albert Bonniot Grenoble France"},{"name":"Universit\u00e9 Joseph Fourier\u2010Grenoble I Institut Albert Bonniot Grenoble France"},{"name":"Plateforme de g\u00e9n\u00e9tique mol\u00e9culaire des cancers, P\u00f4le de Biologie CHU de Grenoble Grenoble France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie C.","family":"B\u00e9n\u00e9","sequence":"additional","affiliation":[{"name":"Immunologie CHU de Nancy & Nancy Universit\u00e9 Vandoeuvre les Nancy France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Formisano\u2010Tr\u00e9ziny","sequence":"additional","affiliation":[{"name":"Facult\u00e9 de M\u00e9decine Universit\u00e9 de la M\u00e9diterran\u00e9e Marseille 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