{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T04:40:16Z","timestamp":1761021616446},"reference-count":50,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2004,9,17]],"date-time":"2004-09-17T00:00:00Z","timestamp":1095379200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2004,10]]},"abstract":"<jats:p><jats:bold>Abstract.\u2002<\/jats:bold> Low\u2010frequency electromagnetic fields are suspected of being involved in carcinogenesis, particularly in processes that could be related to cancer promotion. Because development of cancer is associated with deregulated cell growth and we previously observed a magnetic field\u2010induced decrease in DNA synthesis [Lange <jats:italic>et\u00a0al.<\/jats:italic> (2002) Alterations in the cell cycle and in the protein level of cyclin\u00a0D1p, 21<jats:sup>CIP1<\/jats:sup>, and p16<jats:sup>INK4a<\/jats:sup> after exposure to 50\u00a0HZ. MF in human cells. <jats:italic>Radiat. Environ. Biophys.<\/jats:italic><jats:bold>41<\/jats:bold>, 131], this study aims to document the influence of 50\u00a0Hz, 1\u00a0mT magnetic fields (MF), with or without initial \u03b3\u2010ionizing radiation (IR), on the following cell proliferation\u2010relevant parameters in human amniotic fluid cells (AFC): cell cycle distribution, expression of the G<jats:sub>1<\/jats:sub> phase\u2010regulating proteins Cdk4, cyclin\u00a0D1, p21<jats:sup>CIP1<\/jats:sup> and p16<jats:sup>INK4a<\/jats:sup>, and Cdk4 activity. While IR induced a G<jats:sub>1<\/jats:sub> delay and a dose\u2010dependent G<jats:sub>2<\/jats:sub> arrest, no discernible changes in cell cycle kinetics were observed due to MF exposure. However, a significant decrease in the protein expression of cyclin D1 and an increase in p21<jats:sup>CIP1<\/jats:sup>\u2010 and p16<jats:sup>INK4a<\/jats:sup>\u2010expression could be detected after exposure to MF alone. IR\u2010exposure caused an augmentation of p21<jats:sup>CIP1<\/jats:sup>\u2010 and p16<jats:sup>INK4a<\/jats:sup>\u2010 levels as well, but did not alter cyclin\u00a0D1 expression. A slight diminution of Cdk4 activity was noticed after MF exposure only, indicating that Cdk4 appears not to act as a mediator of MF\u2010 or IR\u2010induced changes in the cell cycle of AFC cells. Co\u2010exposure to MF\/IR affected neither cell cycle distribution nor protein expression or kinase activity additionally or synergistically, and therefore MF seems not to modify the mutagenic potency of IR.<\/jats:p>","DOI":"10.1111\/j.1365-2184.2004.00317.x","type":"journal-article","created":{"date-parts":[[2004,9,17]],"date-time":"2004-09-17T15:53:49Z","timestamp":1095436429000},"page":"337-349","source":"Crossref","is-referenced-by-count":26,"title":["Modifications in cell cycle kinetics and in expression of G<sub>1<\/sub> phase\u2010regulating proteins in human amniotic cells after exposure to electromagnetic fields and ionizing radiation"],"prefix":"10.1111","volume":"37","author":[{"given":"S.","family":"Lange","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Viergutz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Simk\u00f3","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,9,17]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/aje\/152.1.20"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-5371-7_16"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1011297803849"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2001.4423"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4274(03)00111-5"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1995.1356"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.3109\/03008200109016841"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1667\/0033-7587(2003)159[0072:COTMOI]2.0.CO;2"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2001.5501"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90379-4"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.20.13961"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1006\/excr.1997.3496"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.2486\/indhealth.40.320"},{"key":"e_1_2_6_15_1","first-page":"507","article-title":"P16Ink4a tumor suppressor function in lung cancer cells involves cyclin\u2010dependent kinase 2 inhibition by Cip\/Kip protein redistribution","volume":"11","author":"Grimison B","year":"2000","journal-title":"Cell Growth Differ."},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-437X(96)90011-8"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/bem.10062"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/22.7.1043"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.18.9.5284"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.23.13197"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-186X(200002)21:2<122::AID-BEM7>3.0.CO;2-D"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1998.9579"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00411-002-0145-8"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199707033370101"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/20.8.1633"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1202777"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.14.3.2066"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/bem.10019"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.14.3.2077"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1667\/0033-7587(2000)153[0663:EODCTL]2.0.CO;2"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/374131a0"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2744(199809)23:1<1::AID-MC1>3.0.CO;2-Q"},{"key":"e_1_2_6_33_1","first-page":"187","article-title":"Effect of 72 Hz pulsed magnetic field exposure on ras p21 expression in CCRF\u2010CEM cells","volume":"13","author":"Phillips JL","year":"1993","journal-title":"Cancer Biochem. 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