{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T02:59:31Z","timestamp":1791169171115,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2006,11,15]],"date-time":"2006-11-15T00:00:00Z","timestamp":1163548800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:p><jats:bold>Abstract.\u2002<\/jats:bold> We tested the effect of iron deprivation on cell death induction in human Raji cells pre\u2010adapted to differing availability of extracellular iron. Iron deprivation was achieved by incubation in a defined iron\u2010free medium. Original Raji cells have previously been adapted to long\u2010term culture in a defined medium with 5\u00a0\u00b5g\/ml of iron\u2010saturated human transferrin as a source of iron. Raji\/lowFe cells were derived from original Raji cells by subsequent adaptation to culture in the medium with 50\u00a0\u00b5<jats:sc>m<\/jats:sc> ferric citrate as a source of iron. Raji\/lowFe\u2010re cells were derived from Raji\/lowFe cells by re\u2010adaptation to the transferrin\u2010containing (5\u00a0\u00b5g\/ml) medium. Iron deprivation induced cell death in both Raji cells and Raji\/lowFe\u2010re cells; that is, cells pre\u2010adapted to a near optimum source of extracellular iron (5\u00a0\u00b5g\/ml of transferrin). However, Raji\/lowFe cells preadapted to a limited source of extracellular iron (50\u00a0\u00b5<jats:sc>m<\/jats:sc> ferric citrate) became resistant to the induction of cell death by iron deprivation. We demonstrated that cell death induction by iron deprivation in Raji cells correlates with the activation of executioner caspase\u20103 and the cleavage of caspase\u20103 substrate, poly\u2010ADP ribose polymerase. Two other executioner caspases, caspase\u20107 and caspase\u20106, were not activated. Taken together, we suggest that in human Raji cells, iron deprivation induces apoptotic cell death related to caspase\u20103 activation. However, the sensitivity of the cells to death induction by iron deprivation can be reversibly changed by extracellular iron availability. The cells pre\u2010adapted to a limited source of extracellular iron became resistant.<\/jats:p>","DOI":"10.1111\/j.1365-2184.2006.00411.x","type":"journal-article","created":{"date-parts":[[2006,11,16]],"date-time":"2006-11-16T01:24:06Z","timestamp":1163640246000},"page":"551-561","source":"Crossref","is-referenced-by-count":12,"title":["Sensitivity of cells to apoptosis induced by iron deprivation can be reversibly changed by iron availability"],"prefix":"10.1111","volume":"39","author":[{"given":"M.","family":"Koc","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Z.","family":"Nad\u2019ov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Kov\u00e1\u0159","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,11,15]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4827(77)90089-1"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1159\/000203951"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.68.1.383"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.4.1907-1916.2005"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(94)00755-1"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1996.0184"},{"key":"e_1_2_6_8_1","doi-asserted-by":"crossref","first-page":"4109","DOI":"10.4049\/jimmunol.157.9.4109","article-title":"Increased heat shock protein 90 (hsp90) expression leads to increased apoptosis in the monoblastoid cell line U937 following induction with TNF\u2010alpha and cycloheximide: a possible role in immunopathology","volume":"157","author":"Galea\u2010Louri J","year":"1996","journal-title":"J. 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