{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T01:43:43Z","timestamp":1791078223540,"version":"4.1.0"},"reference-count":24,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2007,5,22]],"date-time":"2007-05-22T00:00:00Z","timestamp":1179792000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2007,6]]},"abstract":"<jats:p><jats:bold>Abstract.\u2002<\/jats:bold>  <jats:italic>Objectives<\/jats:italic>: Expression of the nuclear Ki\u201067 protein (pKi\u201067) is strongly associated with cell proliferation. For this reason, antibodies against this protein are widely used as prognostic tools for the assessment of cell proliferation in biopsies from cancer patients. Despite this broad application in histopathology, functional evidence for the physiological role of pKi\u201067 is still missing. Recently, we proposed a function of pKi\u201067 in the early steps of ribosomal RNA (rRNA) synthesis. Here, we have examined the involvement of pKi\u201067 in this process by photochemical inhibition using chromophore\u2010assisted light inactivation (CALI). <jats:italic>Materials and methods<\/jats:italic>: Anti\u2010pKi\u201067 antibodies were labelled with the fluorochrome fluorescein 5(6)\u2010isothiocyanate and were irradiated after binding to their target protein. <jats:italic>Results<\/jats:italic>: Performing CALI <jats:italic>in vitro<\/jats:italic> on cell lysates led to specific cross\u2010linking of pKi\u201067. Moreover, the upstream binding factor (UBF) necessary for rRNA transcription was also partly subjected to cross\u2010link formation, indicating a close spatial proximity of UBF and pKi\u201067. CALI in living cells, using micro\u2010injected antibody, caused a striking relocalization of UBF from foci within the nucleoli to spots located at the nucleolar rim or within the nucleoplasm. pKi\u201067\u2010CALI resulted in dramatic inhibition of RNA polymerase I\u2010dependent nucleolar rRNA synthesis, whereas RNA polymerase II\u2010dependent nucleoplasmic RNA synthesis remained almost unaltered. <jats:italic>Conclusions<\/jats:italic>: Our data presented here argue for a crucial role of pKi\u201067 in RNA polymerase I\u2010dependent nucleolar rRNA synthesis.<\/jats:p>","DOI":"10.1111\/j.1365-2184.2007.00433.x","type":"journal-article","created":{"date-parts":[[2007,5,23]],"date-time":"2007-05-23T20:39:15Z","timestamp":1179952755000},"page":"422-430","source":"Crossref","is-referenced-by-count":128,"title":["Chromophore\u2010assisted light inactivation of pKi\u201067 leads to inhibition of ribosomal RNA synthesis"],"prefix":"10.1111","volume":"40","author":[{"given":"R.","family":"Rahmanzadeh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"H\u00fcttmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Gerdes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Scholzen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,5,22]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M108118200"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/1615-9861(200203)2:3<247::AID-PROT247>3.0.CO;2-K"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3413483"},{"key":"e_1_2_6_5_1","doi-asserted-by":"crossref","first-page":"3199","DOI":"10.1242\/jcs.114.17.3199","article-title":"Revealing the unseen: the organizer region of the nucleolus","volume":"114","author":"Biggiogera M","year":"2001","journal-title":"J. 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Invest"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(76)90068-5"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1098503R"},{"key":"e_1_2_6_14_1","first-page":"301","article-title":"Regulators of nucleolar functions","volume":"5","author":"Hernandez\u2010Verdun D","year":"2003","journal-title":"Prog. Cell Cycle Res"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.15.5454"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.11111"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2004.03.016"},{"key":"e_1_2_6_18_1","first-page":"629","article-title":"New Ki\u201067\u2010equivalent murine monoclonal antibodies (MIB 1\u20133) generated against bacterially expressed parts of the Ki\u201067 cDNA containing three 62 base pair repetitive elements encoding for the Ki\u201067 epitope","volume":"68","author":"Key G","year":"1993","journal-title":"Lab. Invest"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(78)80048-5"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.7.2659"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00327463"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(200003)182:3<311::AID-JCP1>3.0.CO;2-9"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/hon.2900050107"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.8.4293"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi052395a"}],"container-title":["Cell Proliferation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2184.2007.00433.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2184.2007.00433.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T23:18:04Z","timestamp":1697239084000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2184.2007.00433.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,5,22]]},"references-count":24,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2007,6]]}},"alternative-id":["10.1111\/j.1365-2184.2007.00433.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2184.2007.00433.x","archive":["Portico"],"relation":{},"ISSN":["0960-7722","1365-2184"],"issn-type":[{"value":"0960-7722","type":"print"},{"value":"1365-2184","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,5,22]]}}}