{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T04:12:19Z","timestamp":1793938339150,"version":"4.8.0"},"reference-count":54,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2008,7,31]],"date-time":"2008-07-31T00:00:00Z","timestamp":1217462400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2008,10]]},"abstract":"<jats:p><jats:bold>Abstract.\u2003<\/jats:bold>  <jats:italic>Objectives<\/jats:italic>: Adipose tissue is the most abundant and accessible source of adult stem cells. Human processed lipoaspirate contains pre\u2010adipocytes that possess one of the a characteristic pathways of multipotent adult stem cells and are able to differentiate <jats:italic>in vitro<\/jats:italic> into mesenchymal and also neurogenic lineages. Because stem cells have great potential for use in tissue repair and regeneration, it would be significant to be able to obtain large amounts of these cells <jats:italic>in vitro<\/jats:italic>. As demonstrated previously, purine nucleosides and nucleotides mixtures can act as mitogens for several cell types. The aim of this study was to evaluate the effects of polydeoxyribonucleotides (PDRN), at appropriate concentrations, on human pre\u2010adipocytes grown in a controlled medium, also using different passages, so as to investigate the relationship between the effect of this compound and cellular senescence, which is the phenomenon when normal diploid cells lose the ability to divide further. <jats:italic>Materials and methods<\/jats:italic>: Human pre\u2010adipocytes were obtained by liposuction. Cells from different culture passages (P6 and P16) were treated with PDRN at different experimental times. Cell number was evaluated for each sample by direct counting after trypan blue treatment. DNA assay and the 3\u2010(4,5\u2010dimethylthiazol\u20102\u2010yl)\u20102,5\u2010diphenyltetrazolium bromide test were also carried out in all cases. <jats:italic>Results and Conclusions<\/jats:italic>: PDRN seemed to promote proliferation of human pre\u2010adipocytes at both passages, but cell population growth increased in pre\u2010adipocyte at P16, after 9\u00a0days as compared to control. Our data suggest that PDRN could act as a pre\u2010adipocyte growth stimulator.<\/jats:p>","DOI":"10.1111\/j.1365-2184.2008.00547.x","type":"journal-article","created":{"date-parts":[[2008,7,31]],"date-time":"2008-07-31T14:32:22Z","timestamp":1217514742000},"page":"739-754","source":"Crossref","is-referenced-by-count":38,"title":["<i>In vitro<\/i> polydeoxyribonucleotide effects on human pre\u2010adipocytes"],"prefix":"10.1111","volume":"41","author":[{"given":"E.","family":"Raposio","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Guida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Coradeghini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Scanarotti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Parodi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Baldelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Fiocca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.L.","family":"Santi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,9,10]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.PRS.0000055043.62589.05"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/1479-5876-5-55"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0781.2007.00320.x"},{"key":"e_1_2_6_5_1","first-page":"153","article-title":"Treatment of acute radiodermatitis of first and second degrees with semi\u2010greasy placenta ointment","volume":"4","author":"Bigliardi P","year":"1982","journal-title":"Int. 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