{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T23:18:22Z","timestamp":1778887102813,"version":"3.51.4"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,12,16]],"date-time":"2011-12-16T00:00:00Z","timestamp":1323993600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cell Proliferation"],"published-print":{"date-parts":[[2012,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:bold>Objectives:\u2002<\/jats:bold> Recently, plant lectins have attracted great interest due to their various biological activities such as anti\u2010cancer, anti\u2010fungal and anti\u2010viral activities. We have reported earlier concerning anti\u2010proliferation of human cancer cell lines by a galactose\u2010binding lectin (AML), from a Chinese herb, <jats:italic>Astragalus membranaceus<\/jats:italic>. In the present study, detailed investigations into the mechanism of such anti\u2010proliferation properties have been carried out.<\/jats:p><jats:p><jats:bold>Materials and methods:\u2002<\/jats:bold> Mechanism of apoptosis initiation in K562 cells by AML was investigated by morphology, flow cytometry and western blot analysis.<\/jats:p><jats:p><jats:bold>Results:\u2002<\/jats:bold> AML induced apoptosis in a caspase\u2010dependent manner in the chronic myeloid leukemia cell line, K562. Furthermore, we observed that cytotoxicity and apoptosis of K562 cells induced by AML were completely abolished in presence of lactose or galactose.<\/jats:p><jats:p><jats:bold>Conclusions:\u2002<\/jats:bold> Our results suggest that AML could act as a potential anti\u2010cancer drug.<\/jats:p>","DOI":"10.1111\/j.1365-2184.2011.00800.x","type":"journal-article","created":{"date-parts":[[2011,12,16]],"date-time":"2011-12-16T06:30:43Z","timestamp":1324017043000},"page":"15-21","source":"Crossref","is-referenced-by-count":31,"title":["<i>Astragalus membranaceus<\/i> lectin (AML) induces caspase\u2010dependent apoptosis in human leukemia cells"],"prefix":"10.1111","volume":"45","author":[{"given":"L. H.","family":"Huang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Q. J.","family":"Yan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. 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