{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T01:09:42Z","timestamp":1791421782231,"version":"4.3.3"},"reference-count":27,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2004,10,12]],"date-time":"2004-10-12T00:00:00Z","timestamp":1097539200000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2004,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:bold>Background\n\t\t\t\t\t<\/jats:bold> The use of recombinant allergens for the diagnosis and immunotherapy of allergy may offer several advantages over allergen extracts.<\/jats:p><jats:p><jats:bold>Objective\n\t\t\t\t\t<\/jats:bold> To produce recombinant dog allergens Can f 1 and Can f 2 in <jats:italic>Pichia pastoris<\/jats:italic> yeast and to assess their suitability for the diagnosis of dog allergy.<\/jats:p><jats:p><jats:bold>Methods\n\t\t\t\t\t<\/jats:bold> Clinically diagnosed dog\u2010allergic patients' and healthy non\u2010atopic dog owners' reactivities against recombinant Can f 1 and Can f 2 and commercial dog epithelial extract were studied by a panel of methods including skin prick test (SPT), ELISA and IgE immunoblotting.<\/jats:p><jats:p><jats:bold>Results\n\t\t\t\t\t<\/jats:bold> Recombinant Can f 1 and Can f 2 were found immunologically functional: they bound dog\u2010allergic patients' IgE in immunoblotting and inhibited specifically the binding of IgE to their natural counterparts in the dog allergen extract. Moreover, patients' IgE reactivity in immunoblotting to natural Can f 1 and their SPT with the recombinant allergen were perfectly concordant (\u03c6 coefficient 1.0, <jats:italic>P<\/jats:italic>&lt;0.001). The concordance was slightly lower with recombinant Can f 2 (\u03c6 coefficient 0.92, <jats:italic>P<\/jats:italic>&lt;0.001). A lower number of dog\u2010allergic patients, 52%, reacted against Can f 1 than previously reported. About one\u2010third of the patients reacted to Can f 2. In immunoblotting, the highest prevalence of reactivity, 60%, was directed to an 18\u2003kDa component. Aminoterminal sequencing showed this to be a previously unidentified allergenic protein.<\/jats:p><jats:p><jats:bold>Conclusions\n\t\t\t\t\t<\/jats:bold> The recombinant allergens can be used reliably to identify Can f 1 and Can f 2\u2010sensitized individuals. However, the two allergens are insufficient as reagents for diagnosing dog allergy.<\/jats:p>","DOI":"10.1111\/j.1365-2222.2004.02071.x","type":"journal-article","created":{"date-parts":[[2004,10,12]],"date-time":"2004-10-12T09:10:51Z","timestamp":1097572251000},"page":"1576-1582","source":"Crossref","is-referenced-by-count":43,"title":["Assessment of recombinant dog allergens Can f 1 and Can f 2 for the diagnosis of dog 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