{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T18:12:36Z","timestamp":1760897556749},"reference-count":32,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2004,10,12]],"date-time":"2004-10-12T00:00:00Z","timestamp":1097539200000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2004,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:bold>Background\n\t\t\t\t\t<\/jats:bold> We previously reported that ovalbumin\u2013diphtheria toxin (OVA\u2013DT) fusion protein eliminates mast cells bearing OVA\u2010specific IgE and protects OVA\u2010sensitized mice from fatal anaphylaxis induced by OVA challenge.<\/jats:p><jats:p><jats:bold>Objective\n\t\t\t\t\t<\/jats:bold> To prove the specificity of therapeutic effect of OVA\u2013DT to allergy induced by OVA only and not by other allergens such as human serum albumin (HSA), and to examine the cytotoxic effect of OVA\u2013DT on B cells bearing OVA\u2010specific IgE.<\/jats:p><jats:p><jats:bold>Methods\n\t\t\t\t\t<\/jats:bold> Mice were sensitized with two different antigens, OVA and HSA, and then treated with OVA\u2013DT. The therapeutic effect of OVA\u2013DT on the allergy response to each of allergen was evaluated by anaphylactic test. The effect of OVA\u2013DT on the production of allergen\u2010specific Ig isotypes of the sensitized mice and the cytotoxic effect of OVA\u2013DT on B cells expressing OVA\u2010specific IgE were examined.<\/jats:p><jats:p><jats:bold>Results\n\t\t\t\t\t<\/jats:bold> OVA\u2013DT suppressed only OVA\u2010induced allergy but not HSA\u2010induced allergy in mice sensitized with a mixture of OVA and HSA. The suppression was prolonged even to the mice boosted with the same allergen 14 days after last treatment of OVA\u2013DT.<\/jats:p><jats:p>In addition, when the sensitized mice were boosted with the same allergens 14 days after last treatment of OVA\u2013DT, the mice showed to increase the production of OVA\u2010specific IgG2a\/IgG3 and decreased that of OVA\u2010specific IgE. OVA\u2013DT targeted B cells bearing OVA\u2010specific IgE, and killed them by DT\u2010mediated cytotoxicity.<\/jats:p><jats:p><jats:bold>Conclusion\n\t\t\t\t\t<\/jats:bold> The therapeutic effect of OVA\u2013DT was specific to OVA\u2010induced allergy and the suppression of OVA\u2010induced allergy was continuously shown in the mice boosted with the same allergens. This is considered to be caused by the increase of OVA\u2010specific IgG2a and IgG3, and because of the decrease of OVA\u2010specific IgE by killing of B cells bearing OVA\u2010specific IgE.<\/jats:p>","DOI":"10.1111\/j.1365-2222.2004.02077.x","type":"journal-article","created":{"date-parts":[[2004,10,12]],"date-time":"2004-10-12T09:10:51Z","timestamp":1097572251000},"page":"1642-1648","source":"Crossref","is-referenced-by-count":9,"title":["Allergen\u2010specific immunosuppression by ovalbumin fused with diphtheria toxin in mice sensitized with albumins of different origin"],"prefix":"10.1111","volume":"34","author":[{"given":"B. K.","family":"Lee","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. E.","family":"Yoo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y. S.","family":"Jang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. Y.","family":"Kim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. S.","family":"Hong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. 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