{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T16:55:38Z","timestamp":1761843338893},"reference-count":27,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2004,11,12]],"date-time":"2004-11-12T00:00:00Z","timestamp":1100217600000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2004,11]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:bold>Background\n\t\t\t\t\t<\/jats:bold> Our previous studies have presented evidence that lysed <jats:italic>Enterococcus faecalis<\/jats:italic> FK\u201023 (LFK), a lysozyme and heat\u2010treated probiotic product, can inhibit allergen\u2010induced local accumulation of eosinophils in mice.<\/jats:p><jats:p><jats:bold>Objective\n\t\t\t\t\t<\/jats:bold> The purpose of this experimental study was to evaluate the influence of orally administrated LFK on the host immune responses.<\/jats:p><jats:p><jats:bold>Methods\n\t\t\t\t\t<\/jats:bold> BALB\/c mice were sensitized subcutaneously, and challenged intraperitoneally by cedar pollen allergen. Blood and spleen samples were collected after oral administration of LFK 60\u2003mg\/day for 21 days. The serum levels of total and allergen\u2010specific IgE and IgG2a antibodies and the production of IL\u20104, IL\u20105 and IFN\u2010\u03b3 generated by allergen\u2010stimulated cultured splenocytes were determined. Additionally, the effect of LFK on active cutaneous anaphylaxis (ACA) induced by ovalbumin (OVA) challenge in mice was measured after 28 days LFK treatment.<\/jats:p><jats:p><jats:bold>Results\n\t\t\t\t\t<\/jats:bold> No significant differences in serum immunoglobulin levels, as well as in cytokine production of splenocytes were observed between LFK\u2010treated and control mice (<jats:italic>P<\/jats:italic>&gt;0.05). There was, however, an increasing tendency of allergen\u2010specific IgG2a level in mice after LFK treatment for 21 days compared with controls (<jats:italic>P<\/jats:italic>=0.060). Furthermore, the serum ratio of specific IgE to IgG2a was found to be significantly decreased in the LFK group (<jats:italic>P<\/jats:italic>=0.005). In addition, a significant inhibition of OVA\u2010induced ACA reaction was observed in mice that had been fed for 28 days with LFK compared with control mice (<jats:italic>P<\/jats:italic>=0.008).<\/jats:p><jats:p><jats:bold>Conclusion\n\t\t\t\t\t<\/jats:bold> These results suggest that LFK shows an anti\u2010inflammatory effect, which may be part of the mechanism for protection against IgE\u2010mediated allergy.<\/jats:p>","DOI":"10.1111\/j.1365-2222.2004.02092.x","type":"journal-article","created":{"date-parts":[[2004,11,15]],"date-time":"2004-11-15T19:19:36Z","timestamp":1100546376000},"page":"1784-1788","source":"Crossref","is-referenced-by-count":19,"title":["Effects of lysed <i>Enterococcus faecalis<\/i> FK\u201023 on allergen\u2010induced serum antibody responses and active cutaneous anaphylaxis in 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