{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T16:55:41Z","timestamp":1761843341190},"reference-count":37,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2004,12,13]],"date-time":"2004-12-13T00:00:00Z","timestamp":1102896000000},"content-version":"vor","delay-in-days":12,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2004,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:bold>Background\n\t\t\t\t\t<\/jats:bold> Different subsets of dendritic cells (DCs), identified in mouse spleen by their differential expression of CD8\u03b1, can induce different T\u2010helper (Th) responses after systemic administration. CD8\u03b1<jats:sup>\u2212<\/jats:sup> DCs have been shown to preferentially induce Th type 2 (Th2) responses whereas CD8\u03b1<jats:sup>+<\/jats:sup> DCs induce Th1 responses.<\/jats:p><jats:p><jats:bold>Objective\n\t\t\t\t\t<\/jats:bold> To study if these DC subsets can still induce different Th responses in the Th2\u2010prone milieu of the lung and differentially prime for eosinophilic airway inflammation, typical of asthma.<\/jats:p><jats:p><jats:bold>Methods\n\t\t\t\t\t<\/jats:bold> Donor mice first received daily Flt3L injections to expand DC numbers. Purified CD8\u03b1<jats:sup>+<\/jats:sup> or CD8\u03b1<jats:sup>\u2212<\/jats:sup> splenic DCs were pulsed with ovalbumin (OVA) or phosphate\u2010buffered saline and injected intratracheally into recipient mice in which carboxyfluorescein diacetate succinimidyl ester\u2010labelled OVA\u2010specific T cell receptor transgenic T cells had been injected intravenously 2 days earlier. T cell proliferation and cytokine production of Ag\u2010specific T cells were evaluated in the mediastinal lymph nodes (MLNs) 4 days later. The capacity of both subsets of DCs, to prime for eosinophilic airway inflammation was determined by challenging the mice with OVA aerosol 10 days later.<\/jats:p><jats:p><jats:bold>Results\n\t\t\t\t\t<\/jats:bold> CD8\u03b1<jats:sup>\u2212<\/jats:sup> DCs migrated to the MLN and induced a vigorous proliferative T cell response accompanied by high\u2010level production of IL\u20104, IL\u20105, IL\u201010 and also IFN\u2010\u03b3 during the primary response and during challenge with aerosol, leading to eosinophilic airway inflammation. In the absence of migration to the MLN, CD8\u03b1<jats:sup>+<\/jats:sup> DCs still induced a proliferative response with identical levels of IFN\u2010\u03b3 but reduced Th2 cytokines compared with CD8\u03b1<jats:sup>\u2212<\/jats:sup> DCs, which led to weak eosinophilic airway inflammation upon OVA aerosol challenge. Unpulsed DCs did not induce proliferation or cytokine production in Ag\u2010specific T cells.<\/jats:p><jats:p><jats:bold>Conclusion\n\t\t\t\t\t<\/jats:bold> CD8\u03b1<jats:sup>\u2212<\/jats:sup> DCs are superior compared with CD8\u03b1<jats:sup>+<\/jats:sup> DCs in inducing Th2 responses and eosinophilic airway inflammation in the Th2\u2010prone environment of the lung.<\/jats:p>","DOI":"10.1111\/j.1365-2222.2004.02133.x","type":"journal-article","created":{"date-parts":[[2004,12,13]],"date-time":"2004-12-13T11:16:00Z","timestamp":1102936560000},"page":"1834-1840","source":"Crossref","is-referenced-by-count":32,"title":["Differential capacity of CD8\u03b1<sup>+<\/sup> or CD8\u03b1<sup>\u2212<\/sup> dendritic cell subsets to prime for eosinophilic airway inflammation in the T\u2010helper type 2\u2010prone milieu of the lung"],"prefix":"10.1111","volume":"34","author":[{"given":"H.","family":"Hammad","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V. C.","family":"De Vries","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Maldonado\u2010Lopez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Moser","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Maliszewski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. C.","family":"Hoogsteden","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. N.","family":"Lambrecht","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2004,12,13]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/35037009"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI8107"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.160.8.4090"},{"key":"e_1_2_6_5_2","first-page":"421","article-title":"Regulation of immunologic homeostasis in peripheral tissues by dendritic cells","volume":"105","author":"Holt PG","year":"2000","journal-title":"the respiratory tract as a paradigm"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(02)00276-5"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.188.11.2019"},{"key":"e_1_2_6_8_2","first-page":"840","article-title":"Site of antigen delivery can influence T cell priming","volume":"30","author":"Constant S","year":"2000","journal-title":"pulmonary environment promotes preferential Th2-type differentiation"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI0216109"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/79734"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.189.3.587"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.3.1036"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.8.4345"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.69.6.951"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/immu.200390001"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.283.5405.1183"},{"key":"e_1_2_6_17_2","first-page":"311","article-title":"Kinetics of dendritic cell activation","volume":"1","author":"Langenkamp A","year":"2000","journal-title":"impact on priming of Th1, Th2 and nonpolarized T cells"},{"key":"e_1_2_6_18_2","first-page":"405","article-title":"Human monocyte\u2010derived dendritic cells induce naive T cell differentiation into T helper cell type 2 (Th2) or Th1\/Th2 effectors","volume":"192","author":"Tanaka H","year":"2000","journal-title":"role of stimulator\/responder ratio"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.4.1704"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.9.5448"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-03-0673"},{"key":"e_1_2_6_22_2","first-page":"214","article-title":"Regulation of T cell cytokine production by dendritic cells Immunol","volume":"78","author":"Kronin V","year":"2000","journal-title":"Cell Biol"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.189.12.1981"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.6.2937"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-03-0673"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.20.6.3484"},{"key":"e_1_2_6_27_2","first-page":"1524","article-title":"Monocyte\u2010derived dendritic cells induce a house dust mite\u2010specific Th2 allergic inflammation in the lung of humanized SCID mice","volume":"169","author":"Hammad H","year":"2002","journal-title":"involvement of CCR7"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.189.3.587"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0604325"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1002\/1521-4141(200001)30:1<316::AID-IMMU316>3.0.CO;2-0"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI4160"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.189.3.593"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-4141(199805)28:05<1636::AID-IMMU1636>3.0.CO;2-9"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni854"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20020161"},{"key":"e_1_2_6_36_2","first-page":"2081","article-title":"Flt3 ligand","volume":"1","author":"Agrawal DK","year":"2001","journal-title":"a novel cytokine prevents allergic asthma in a mouse model"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.193.1.51"},{"key":"e_1_2_6_38_2","doi-asserted-by":"crossref","first-page":"1310","DOI":"10.4049\/jimmunol.162.3.1310","article-title":"Comparison of lung dendritic cells and B cells in stimulating naive antigen\u2010specific T cells","volume":"162","author":"Masten BJ","year":"1999","journal-title":"J Immunol"}],"container-title":["Clinical &amp; Experimental Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2222.2004.02133.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2222.2004.02133.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,14]],"date-time":"2023-10-14T00:51:15Z","timestamp":1697244675000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2222.2004.02133.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,12]]},"references-count":37,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2004,12]]}},"alternative-id":["10.1111\/j.1365-2222.2004.02133.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2222.2004.02133.x","archive":["Portico"],"relation":{},"ISSN":["0954-7894","1365-2222"],"issn-type":[{"value":"0954-7894","type":"print"},{"value":"1365-2222","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,12]]}}}