{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T01:18:23Z","timestamp":1771550303839,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,12,22]],"date-time":"2005-12-22T00:00:00Z","timestamp":1135209600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2006,1]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:bold>Background\n\t\t\t\t\t<\/jats:bold> Second\u2010generation antihistamines are H<jats:sub>1<\/jats:sub> receptor antagonists and may have additional anti\u2010inflammatory effects.<\/jats:p><jats:p><jats:bold>Objective\n\t\t\t\t\t<\/jats:bold> The aims of the study were to evaluate the effect of desloratadine (DL) on cytokine secretion by epithelial cells from both nasal mucosa (NM) and polyps (NP), and on eosinophil survival primed by epithelial cell secretions.<\/jats:p><jats:p><jats:bold>Methods\n\t\t\t\t\t<\/jats:bold> Epithelial cells were cultured and stimulated with fetal bovine serum (FBS), IL\u20101\u03b2 or TNF\u2010\u03b1 with and without DL for 24\u2003h. Culture supernatant cytokines concentration were measured by ELISA. Peripheral blood eosinophils were incubated with human epithelial cell conditioned media (HECM) and DL. Eosinophil survival was assessed by Trypan blue dye exclusion. Results are expressed as mean\u00b1SEM of cytokine concentration (pg\/mL) or eosinophil survival index (%).<\/jats:p><jats:p><jats:bold>Results\n\t\t\t\t\t<\/jats:bold> FBS increased granulocyte\u2010macrophage colony\u2010stimulating factor (GM\u2010CSF), vascular endothelial growth factor (VEGF), IL\u20106, IL\u20108, and TGF\u2010\u03b2<jats:sub>1<\/jats:sub> secretion in epithelial cell cultures from both NM and NP. Only GM\u2010CSF secretion was significantly (<jats:italic>P<\/jats:italic>&lt;0.05) inhibited by a dose\u2010response of DL compared with positive controls, in both NM (10<jats:sup>\u22125<\/jats:sup>\u2003<jats:sc>m<\/jats:sc>: 125\u00b136\u2003pg\/mL, 10<jats:sup>\u22126<\/jats:sup>\u2003<jats:sc>m<\/jats:sc>: 95\u00b122\u2003pg\/mL vs. control: 256\u00b191\u2003pg\/mL, <jats:italic>n<\/jats:italic>=6) and NP (10<jats:sup>\u22125<\/jats:sup>\u2003<jats:sc>m<\/jats:sc>: 80\u00b129\u2003pg\/mL, 10<jats:sup>\u22126<\/jats:sup>\u2003<jats:sc>m<\/jats:sc>: 109\u00b145\u2003pg\/mL vs. control: 333\u00b1212\u2003pg\/mL, <jats:italic>n<\/jats:italic>=6). DL also showed an inhibitory effect on HECM\u2010induced eosinophil survival from both NM and NP. At 72\u2003h, DL significantly (<jats:italic>P<\/jats:italic>&lt;0.01) inhibited eosinophil survival induced by HECM from NM (10<jats:sup>\u22125<\/jats:sup>\u2003<jats:sc>m<\/jats:sc>: 19.9\u00b15.5%, <jats:italic>n<\/jats:italic>=9; 10<jats:sup>\u22126<\/jats:sup>\u2003<jats:sc>m<\/jats:sc>: 28.7\u00b17.7%, <jats:italic>n<\/jats:italic>=9) and NP (10<jats:sup>\u22125<\/jats:sup>\u2003<jats:sc>m<\/jats:sc>: 6.2\u00b12.8%, <jats:italic>n<\/jats:italic>=11) compared with HECM alone (NM: 42.1\u00b17.3%; NP: 45.3\u00b18.1%).<\/jats:p><jats:p><jats:bold>Conclusion\n\t\t\t\t\t<\/jats:bold> The inhibitory effects of DL on epithelial cell GM\u2010CSF secretion and on eosinophil survival induced by epithelial cell secretions, suggest that this H<jats:sub>1<\/jats:sub> antagonist may regulate eosinophil inflammation in upper airways.<\/jats:p>","DOI":"10.1111\/j.1365-2222.2005.02403.x","type":"journal-article","created":{"date-parts":[[2005,12,22]],"date-time":"2005-12-22T15:47:45Z","timestamp":1135266465000},"page":"52-58","source":"Crossref","is-referenced-by-count":30,"title":["Effect of desloratadine on epithelial cell granulocyte\u2010macrophage colony\u2010stimulating factor secretion and eosinophil survival"],"prefix":"10.1111","volume":"36","author":[{"given":"J.","family":"Mullol","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Roca\u2010Ferrer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Alobid","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L.","family":"Pujols","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Valero","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Xaubet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Bernal\u2010Sprekelsen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Picado","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,22]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-3038.1999.00036.x"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0385-8146(96)00034-X"},{"key":"e_1_2_6_4_2","first-page":"616","article-title":"Eosinophils in nasal polyps and nasal mucosa","volume":"91","author":"Stoop AE","year":"1993","journal-title":"an immunohistochemical study"},{"key":"e_1_2_6_5_2","first-page":"1432","article-title":"Effects of topical anti\u2010inflammatory drugs on eosinophil survival primed by epithelial cells","volume":"27","author":"Mullol J","year":"1997","journal-title":"additive effect of glucocorticoids and nedocromil sodium"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.149.2.8306027"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-3038.2000.00051.x"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.17.6.2865"},{"key":"e_1_2_6_9_2","first-page":"367","article-title":"Interleukin\u20101\u03b2, interleukin\u20105, interleukin\u20106, interleukin\u20108, and tumor necrosis factor\u2010alpha in chronic sinusitis","volume":"14","author":"Lennard CM","year":"2000","journal-title":"response to systemic corticosteroids"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.1995.tb01108.x"},{"key":"e_1_2_6_11_2","first-page":"279","article-title":"Nasal polyposis","volume":"14","author":"Bachert C","year":"2000","journal-title":"from cytokines to growth"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-3003.2000.15b24.x"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.153.5.8630619"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(97)70302-6"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.3950\/jibiinkoka.103.829"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1136\/thx.50.3.270"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.97.10071489"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1398-9995.2003.02172.x"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1053\/rmed.1999.0756"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1053\/rmed.2001.1061"},{"key":"e_1_2_6_21_2","first-page":"97","article-title":"Effects of antihistamines on leukotriene and cytokine release from dispersed nasal polyp cells","volume":"52","author":"Kusters S","year":"2002","journal-title":"Arzneimittelforschung"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.1996.tb04622.x"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1398-9995.2002.23452.x"},{"key":"e_1_2_6_24_2","first-page":"373","article-title":"Effect of loratadine on human eosinophil function in vitro","volume":"71","author":"Eda R","year":"1993","journal-title":"Ann Allergy"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.1997.tb01155.x"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(02)02049-6"},{"key":"e_1_2_6_27_2","first-page":"13","article-title":"Therapeutic points of intervention and clinical implications","volume":"57","author":"Bachert C.","year":"2002","journal-title":"role of desloratadine"},{"key":"e_1_2_6_28_2","unstructured":"AgrawalDK BerroAI.. Effect of desloratadine on the apoptosis of human blood eosinophils. Abstract book of the XXII Congress of the European Academy of Allergology and Clinical Immunology 2003 abstract 955."},{"key":"e_1_2_6_29_2","first-page":"87","article-title":"Proinflammatory cytokines and eosinophil cationic protein on glandular secretion from human nasal mucosa","volume":"108","author":"Roca\u2010Ferrer J","year":"2001","journal-title":"regulation by corticosteroids"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.1999.00630.x"},{"key":"e_1_2_6_31_2","first-page":"330","article-title":"Expression and generation of interleukin\u20108, IL\u20106 and granulocyte\u2010macrophage colony\u2010stimulating factor by bronchial epithelial cells and enhancement by IL\u20101\u03b2 and TNF\u2010\u03b1","volume":"77","author":"Cromwell O","year":"1992","journal-title":"Immunology"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(99)70119-3"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1006\/pupt.1998.0151"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0625.2000.009002118.x"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.2001.01130.x"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1081-1206(10)63219-X"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2003.136"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.1999.00598.x"}],"container-title":["Clinical &amp; Experimental Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2222.2005.02403.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T08:57:20Z","timestamp":1697187440000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2222.2005.02403.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,12,22]]},"references-count":37,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2006,1]]}},"alternative-id":["10.1111\/j.1365-2222.2005.02403.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2222.2005.02403.x","archive":["Portico"],"relation":{},"ISSN":["0954-7894","1365-2222"],"issn-type":[{"value":"0954-7894","type":"print"},{"value":"1365-2222","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,12,22]]}}}