{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T17:13:33Z","timestamp":1791393213997,"version":"4.3.3"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,12,22]],"date-time":"2005-12-22T00:00:00Z","timestamp":1135209600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2006,1]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:bold>Background\n\t\t\t\t\t<\/jats:bold> Immunoglobulin (Ig) E plays a key role in the pathogenesis of atopic diseases, such as asthma, atopic dermatitis and allergic rhinitis. Oral administration of pulverized Konjac glucomannan (PKGM) has recently been demonstrated to prevent both plasma IgE elevation and developing dermatitis in NC\/Nga mice, a model of atopic dermatitis.<\/jats:p><jats:p><jats:bold>Objective\n\t\t\t\t\t<\/jats:bold> To clarify the direct effect of PKGM on the increase of plasma IgE, we employed the system of BALB\/c mouse that increases IgE level without developing dermatitis in response to continuous injection of the extract of syngeneic keratinocytes, PAM 212 cells (PAM extract).<\/jats:p><jats:p><jats:bold>Methods\n\t\t\t\t\t<\/jats:bold> Three weeks after the start of feeding with either control or PKGM diet, mice were injected subcutaneously with PAM extract bi\u2010weekly for 10 weeks. The levels of plasma Igs were measured by enzyme\u2010linked immunosorbent assay every 2 weeks after the injection. The levels of \u025b germline transcription and the amounts of mRNA for IL\u20104, IFN\u2010\u03b3, GATA\u20103 and T bet gene in the spleen were evaluated by real\u2010time RT\u2010PCR at the end of the experiment.<\/jats:p><jats:p><jats:bold>Results\n\t\t\t\t\t<\/jats:bold> On the one hand, PKGM prevented the increase of plasma IgE and IgG (IgG1, IgG2b) induced by PAM extract, and on the other hand, it enhanced the levels of plasma IgG3. However, it did not affect the level of plasma IgM. PKGM also reduced the levels of plasma ovalbumin (OVA)\u2010specific IgE in OVA\u2010sensitized mice. Moreover, PKGM attenuated the induction of \u025b germline transcription and expression levels of mRNA for IL\u20104, IFN\u2010\u03b3 and GATA\u20103 in the spleen of PAM extract\u2010injected mice. PKGM also attenuated the induction of \u025b germline transcription and mRNA for IFN\u2010\u03b3 and T bet in the spleen of phosphate\u2010buffered saline\u2010injected control mice.<\/jats:p><jats:p><jats:bold>Conclusions\n\t\t\t\t\t<\/jats:bold> These results suggested that oral administration of PKGM prevents the elevation of plasma IgE by suppressing IgE class switching in B cells and\/or the commitment development of naive lymphocytes to both T\u2010helper type 1 (Th1) and Th2.<\/jats:p>","DOI":"10.1111\/j.1365-2222.2005.02405.x","type":"journal-article","created":{"date-parts":[[2005,12,22]],"date-time":"2005-12-22T15:47:45Z","timestamp":1135266465000},"page":"102-110","source":"Crossref","is-referenced-by-count":35,"title":["Oral administration of pulverized Konjac glucomannan prevents the increase of plasma immunoglobulin E and immunoglobulin G levels induced by the injection of syngeneic keratinocyte extracts in BALB\/c mice"],"prefix":"10.1111","volume":"36","author":[{"given":"S.","family":"Oomizu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Onishi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Ueda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Mochizuki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Morimoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Kawamoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K.","family":"Ono","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Kameyoshi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Hide","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,12,22]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"302","article-title":"Atopic 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