{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T15:00:45Z","timestamp":1792422045610,"version":"4.5.0"},"reference-count":51,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,2,24]],"date-time":"2006-02-24T00:00:00Z","timestamp":1140739200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2006,3]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:bold>Background\n\t\t\t\t\t<\/jats:bold> Stem cell factor (SCF) is a major mast cell growth factor promoting differentiation, chemotaxis as well as inhibition of apoptosis of mast cells. Regulation of SCF expression by glucocorticoids has not yet been reported in human asthmatic bronchi.<\/jats:p><jats:p><jats:bold>Objective\n\t\t\t\t\t<\/jats:bold> To evaluate SCF mRNA and protein expression in biopsy specimen and bronchoalveolar lavage fluid, respectively, and to determine the mast cell numbers in biopsy sections from control and asthmatic subjects treated or not with glucocorticoids.<\/jats:p><jats:p><jats:bold>Methods\n\t\t\t\t\t<\/jats:bold> Volunteers were recruited out of pollen season. Asthmatic patients were allergic to common allergen extracts including grass and tree pollen, cat, dog or mite; three volunteers had non\u2010allergic asthma. Mast cell numbers were counted after anti\u2010human tryptase immunolabelling. SCF mRNA was quantified by real\u2010time fluorescent PCR (LightCycler<jats:sup>\u2122<\/jats:sup>) after reverse transcription, and SCF protein was measured by ELISA.<\/jats:p><jats:p><jats:bold>Results\n\t\t\t\t\t<\/jats:bold> Asthmatic patients not treated with glucocorticoids showed a 5.8\u2010, 1.8\u2010 and 3.1\u2010fold significant increase in SCF mRNA, protein levels and mast cell numbers, respectively, compared with healthy volunteers of the control group (7.8 and 1.3\u2003pg\/\u03bcg SCF mRNA\/GAPDH; 99.8\u00b111.5 and 56.0\u00b111.0\u2003pg\/mL SCF protein; 103\u00b121 and 33\u00b18 mast cells\/mm<jats:sup>2<\/jats:sup>, respectively; <jats:italic>P<\/jats:italic>&lt;0.05). In asthmatic patients treated with glucocorticoids, a significant decrease of SCF mRNA, protein levels and mast cell numbers was observed as compared with untreated asthmatic patients (1.1\u2003pg\/\u03bcg mRNA; 62.0\u00b110.3\u2003pg\/mL SCF protein and 39\u00b113 mast cells\/mm<jats:sup>2<\/jats:sup>; <jats:italic>P<\/jats:italic>&lt;0.05), reaching levels comparable to those of the control group.<\/jats:p><jats:p><jats:bold>Conclusion\n\t\t\t\t\t<\/jats:bold> Our study shows that SCF is expressed in the bronchus in humans <jats:italic>in vivo<\/jats:italic>. This expression is increased in asthma, and is parallel to the increased mast cell numbers in the airways. Both increases were normalized in glucocorticoid\u2010treated patients, strongly suggesting an involvement of SCF in the mast cell\u2010associated asthmatic disease.<\/jats:p>","DOI":"10.1111\/j.1365-2222.2006.02431.x","type":"journal-article","created":{"date-parts":[[2006,2,24]],"date-time":"2006-02-24T09:53:53Z","timestamp":1140774833000},"page":"317-324","source":"Crossref","is-referenced-by-count":24,"title":["Effect of glucocorticoids on stem cell factor expression in human asthmatic bronchi"],"prefix":"10.1111","volume":"36","author":[{"given":"C. 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