{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T02:59:43Z","timestamp":1792119583326,"version":"4.4.0"},"reference-count":37,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,4,26]],"date-time":"2006-04-26T00:00:00Z","timestamp":1146009600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2006,5]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:bold>Background\n\t\t\t\t\t<\/jats:bold> In contrast to other Rosaceae fruit, only few cases of patients with adverse reactions to strawberry are listed in literature.<\/jats:p><jats:p><jats:bold>Objective\n\t\t\t\t\t<\/jats:bold> To identify allergenic proteins in strawberry and to express and characterize recombinant strawberry lipid transfer protein (LTP; rFra a 3).<\/jats:p><jats:p><jats:bold>Methods\n\t\t\t\t\t<\/jats:bold> Established apple\u2010allergic patients were recruited on the basis of a reported allergic reaction to strawberry (<jats:italic>n<\/jats:italic>=28, confirmed by double\u2010blind placebo\u2010controlled food challenge in four patients) or on the basis of IgE reactivity to LTP (<jats:italic>n<\/jats:italic>=34). Sensitization to purified natural and recombinant allergens was assessed by RAST, immunoblot (inhibition) and basophil histamine release (BHR). A strawberry cDNA library was screened for genes homologous to known fruit allergens. Fra a 3 was cloned and expressed in the yeast <jats:italic>Pichia pastoris<\/jats:italic> and compared with peach and apple LTP by RAST, immunoblot\u2010inhibition and BHR tests.<\/jats:p><jats:p><jats:bold>Results\n\t\t\t\t\t<\/jats:bold> Genes homologous to Bet v 1, Bet v 6, profilin and LTP were identified in a strawberry cDNA library. In BHR the rFra a 3 induced histamine release at a 100\u2010fold higher concentration than peach LTP. RAST inhibition showed high cross\u2010reactivity to peach and apple LTP, although IgE reactivity was lower by a factor 5. On strawberry immunoblot, patients' IgE showed reactivity to a Bet v 1 homologue, profilin, LTP and high\u2010molecular weight bands.<\/jats:p><jats:p><jats:bold>Conclusion\n\t\t\t\t\t<\/jats:bold> In addition to a Bet v 1 homologue, strawberry also contains IgE\u2010binding profilin and LTP. The rFra a 3 has less allergenic potency than peach and apple LTP, and therefore is an interesting tool for future immunotherapy. Fra a 3 does not seem to be clinically relevant.<\/jats:p>","DOI":"10.1111\/j.1365-2222.2006.02453.x","type":"journal-article","created":{"date-parts":[[2006,4,28]],"date-time":"2006-04-28T01:46:10Z","timestamp":1146188770000},"page":"666-675","source":"Crossref","is-referenced-by-count":50,"title":["The role of profilin and lipid transfer protein in strawberry allergy in the Mediterranean area"],"prefix":"10.1111","volume":"36","author":[{"given":"L.","family":"Zuidmeer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Salentijn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. F.","family":"Rivas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. G.","family":"Mancebo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Asero","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. I.","family":"Matos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. T. B.","family":"Pelgrom","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.J.W.J.","family":"Gilissen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Van Ree","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,4,26]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"946","article-title":"Reactivity to potential cross\u2010reactive foods in fruit\u2010allergic patients","volume":"57","author":"Crespo JF","year":"2002","journal-title":"implications for prescribing food avoidance"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1097\/00130832-200206000-00013"},{"key":"e_1_2_6_4_2","first-page":"962","article-title":"Identification of allergens in fruits and vegetables","volume":"95","author":"Ebner C","year":"1995","journal-title":"IgE cross-reactivities with the important birch pollen allergens Bet v 1 and Bet v 2 (birch profilin)"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.1987.tb02225.x"},{"key":"e_1_2_6_6_2","first-page":"289","volume-title":"Contact Urticaria Syndrome","author":"Van Ree R.","year":"1997"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2000.106927"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.0014-2956.2001.02463.x"},{"key":"e_1_2_6_9_2","first-page":"338","article-title":"True multifood allergy in a 4\u2010year\u2010old child","volume":"30","author":"Pajno GB","year":"2002","journal-title":"a case study"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.1996.tb02149.x"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.1995.tb00812.x"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0536.1985.tb02538.x"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.2004.00585.x"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(97)70265-3"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(99)70480-X"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(99)70479-3"},{"key":"e_1_2_6_17_2","first-page":"20","article-title":"Lipid transfer protein","volume":"122","author":"Asero R","year":"2000","journal-title":"a pan-allergen in plant-derived foods that is highly resistant to pepsin digestion"},{"key":"e_1_2_6_18_2","first-page":"436","article-title":"The SAFE project","volume":"60","author":"Hoffmann\u2010Sommergruber K.","year":"2005","journal-title":"plant food allergies field to table strategies for reducing their incidence in Europe an EC-funded study"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(03)02016-5"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.12.5.647"},{"key":"e_1_2_6_21_2","first-page":"3381","article-title":"SWISS\u2010MODEL","volume":"31","author":"Schwede T","year":"2003","journal-title":"an automated protein homology-modeling server"},{"key":"e_1_2_6_22_2","first-page":"2714","article-title":"SWISS\u2010MODEL and the Swiss\u2010PdbViewer","volume":"18","author":"Guex N","year":"1997","journal-title":"an environment for comparative protein modeling"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0795-658"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.1980.tb02020.x"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/0091-6749(81)90133-0"},{"key":"e_1_2_6_26_2","first-page":"1187","article-title":"Allergy to jackfruit","volume":"59","author":"Bolhaar ST","year":"2004","journal-title":"a novel example of Bet v 1-related food allergy"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1159\/000049524"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(75)90025-3"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00021820"},{"key":"e_1_2_6_30_2","first-page":"101","volume-title":"Biogene Amine in Fruchtsaellen","author":"Maxa E","year":"1993"},{"key":"e_1_2_6_31_2","volume-title":"Eetwaar = eetbaar: Over voedsel en allergie","author":"Kamsteeg J","year":"1988"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0963-9969(96)00066-X"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.1978.tb01533.x"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(97)70245-8"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(03)01605-1"},{"key":"e_1_2_6_36_2","first-page":"344","article-title":"Biological properties of plant flavonoids","volume":"34","author":"Middleton E.","year":"1996","journal-title":"an overview"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M202065200"},{"key":"e_1_2_6_38_2","first-page":"3389","article-title":"Gapped BLAST and PSI\u2010BLAST","volume":"25","author":"Altschul SF","year":"1997","journal-title":"a new generation of protein database search programs"}],"container-title":["Clinical &amp; Experimental Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2222.2006.02453.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2222.2006.02453.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T09:36:23Z","timestamp":1697189783000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2222.2006.02453.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,4,26]]},"references-count":37,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2006,5]]}},"alternative-id":["10.1111\/j.1365-2222.2006.02453.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2222.2006.02453.x","archive":["Portico"],"relation":{},"ISSN":["0954-7894","1365-2222"],"issn-type":[{"value":"0954-7894","type":"print"},{"value":"1365-2222","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,4,26]]}}}