{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T04:11:16Z","timestamp":1791173476134,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,3,29]],"date-time":"2006-03-29T00:00:00Z","timestamp":1143590400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2006,5]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:bold>Background\n\t\t\t\t\t<\/jats:bold> Ragweed and mugwort have nearly identical flowering periods. Clinical and serological studies showed that ragweed and mugwort sensitization are often associated and this poses relevant clinical problems in patients for whom specific immunotherapy is warranted.<\/jats:p><jats:p><jats:bold>Objective\n\t\t\t\t\t<\/jats:bold> To establish whether the concurrent ragweed and mugwort pollen hypersensitivity is the result of co\u2010sensitization or of co\u2010recognition by using purified recombinant allergens.<\/jats:p><jats:p><jats:bold>Methods\n\t\t\t\t\t<\/jats:bold> Sensitization to ragweed and mugwort pollen was assessed by skin prick test (SPT) in all patients reporting allergic symptoms in August and September. IgE reactivity of sera from 42 patients (26 Amb+\/Art+, 14 Amb+\/Art\u2212, and two Amb\u2212\/Art+) to ragweed and mugwort pollen extract as well as to several recombinant ragweed (rAmb a 1, rAmb a 5, rAmb a 6, rAmb a 8, rAmb a 9, and Amb a 10) and mugwort (rArt v 1, rArt v 4, rArt v 5, rArt v 6, and three EF\u2010hand calcium\u2010binding protein) allergens was detected by dot\u2010blot and ELISA analyses.<\/jats:p><jats:p><jats:bold>Results\n\t\t\t\t\t<\/jats:bold> IgE reactivity of 372 weed pollen\u2010allergic patients was studied. Mugwort reactivity was strongly associated with ragweed hypersensitivity: only 10\/147 (7%) mugwort\u2010hypersensitive patients were not sensitized to ragweed, whereas 225\/362 (62%) ragweed\u2010hypersensitive patients were not sensitized to mugwort. <jats:italic>In vitro<\/jats:italic>, 90% of ragweed\u2010allergic patients reacted with rAmb a 1. Reactivity to other ragweed allergens ranged between 20% and 35%. Forty\u2010six percent of the mugwort\u2010sensitized patients recognized rArt v 1%, 25% reacted to Art v 4, Art v 5, and Art v 6, and 7% recognized the three\u2010EF hand calcium\u2010binding protein. Immunoblot inhibition experiments showed that pre\u2010incubation with ragweed pollen extract only weakly decreased IgE reactivity to mugwort allergens.<\/jats:p><jats:p><jats:bold>Conclusion\n\t\t\t\t\t<\/jats:bold> Patients showing both ragweed\u2010 and mugwort\u2010positive SPT and\/or RAST are co\u2010sensitized. Future studies will establish whether IgE reactivity translates into clinical symptoms and, hence, if co\u2010sensitized patients should undergo specific immunotherapy with extracts of both mugwort and ragweed pollen.<\/jats:p>","DOI":"10.1111\/j.1365-2222.2006.02477.x","type":"journal-article","created":{"date-parts":[[2006,3,29]],"date-time":"2006-03-29T14:57:10Z","timestamp":1143644230000},"page":"658-665","source":"Crossref","is-referenced-by-count":79,"title":["<i>Artemisia<\/i> and <i>Ambrosia<\/i> hypersensitivity: co\u2010sensitization or co\u2010recognition?"],"prefix":"10.1111","volume":"36","author":[{"given":"R.","family":"Asero","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Wopfner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Gruber","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Gadermaier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Ferreira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,3,29]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"99","article-title":"Pollinosi da Ambrosia artemisiifolia in provincia di Milano","volume":"37","author":"Bottero P","year":"1990","journal-title":"Folia Allergol Immunol Clin"},{"key":"e_1_2_6_3_2","first-page":"241","article-title":"Studio multicentrico sulla prevalenza delle pollinosi nella citt\u00e0 e nella provincia di Milano: 1990\u201395 [Abstract]","volume":"8","author":"Asero R","year":"1998","journal-title":"Giorn It Allergol Immunol Clin"},{"key":"e_1_2_6_4_2","first-page":"125","volume-title":"Allergenic pollen and pollinosis in Europe","author":"Jager S.","year":"1991"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02992997"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.3349\/ymj.1989.30.4.346"},{"key":"e_1_2_6_7_2","first-page":"103","article-title":"Indagine sul contributo di diversi test in vivo e in vitro alla diagnosi differenziale tra pollinosi da Artemisia e da Ambrosia","volume":"4","author":"Asero R","year":"1994","journal-title":"Giorn It Allergol Immunol Clin"},{"key":"e_1_2_6_8_2","first-page":"461","article-title":"Compositae, Artemisia","volume":"1","author":"Caramiello R.","year":"1991","journal-title":"Giorn It Allergol Immunol Clin"},{"key":"e_1_2_6_9_2","first-page":"467","article-title":"Compositae, Ambrosia","volume":"1","author":"Gallesio MT.","year":"1991","journal-title":"Giorn It Allergol Immunol Clin"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0091-6749(98)70384-7"},{"key":"e_1_2_6_11_2","unstructured":"MutschlechnerS MariA Van ReeRet al.Patterns of Art v 1 recognition in muwort and ragweed\u2010sensitized patients. 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