{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T06:25:40Z","timestamp":1778567140068,"version":"3.51.4"},"reference-count":40,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2006,6,1]],"date-time":"2006-06-01T00:00:00Z","timestamp":1149120000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2006,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:bold>Background\n\t\t\t\t\t<\/jats:bold> Approximately 40% of chronic idiopathic urticaria (CIU) subjects have autoantibodies to either Fc\u025bRI\u03b1 or IgE. The effect of such autoantibodies on circulating basophil activation status is unknown.<\/jats:p><jats:p><jats:bold>Objective\n\t\t\t\t\t<\/jats:bold> The expression of cell surface activation markers on basophils from CIU, non\u2010allergic, and allergic subjects were compared. Further, the relationship between marker expression and serum factors reported in CIU, such as histamine\u2010releasing activity (HRA) and immunoreactivity to Fc\u025bRI\u03b1 were examined.<\/jats:p><jats:p><jats:bold>Methods\n\t\t\t\t\t<\/jats:bold> Peripheral blood was obtained from CIU, allergic, and non\u2010allergic donors and fractionated by density gradients. Enriched basophils (1\u201312%) were analysed by flow cytometry for expression of activation markers including CD63, CD69, and CD203c. Dilutions of serum (5\u201350%) were analysed for HRA on basophils from a normal donor. Serum was tested for immunoreactivity by western blotting to a standard cell lysate prepared from an RBL\u2010SX38 cell line transfected with human Fc\u025bRI\u03b1.<\/jats:p><jats:p><jats:bold>Results\n\t\t\t\t\t<\/jats:bold> CIU subjects (<jats:italic>n<\/jats:italic>=9) and allergic subjects (<jats:italic>n<\/jats:italic>=8) exhibited enhanced expression of CD63 and CD69, as compared with non\u2010allergic subjects (<jats:italic>n<\/jats:italic>=7); however, no difference was seen among groups for CD203c expression. Five CIU and two non\u2010allergic subjects had evidence of significant serum HRA (&gt;20%), whereas two CIU, two allergic, and three non\u2010allergic subjects had evidence of serum immunoreactivity to Fc\u025bRI\u03b1. Serum HRA and serum immunoreactivity to Fc\u025bRI\u03b1 were not associated with enhanced surface marker expression.<\/jats:p><jats:p><jats:bold>Conclusion\n\t\t\t\t\t<\/jats:bold> Basophil activation marker expression is increased in CIU subjects and is not associated with serum factors. In addition, serum HRA and Fc\u025bRI\u03b1 immunoreactivity are not unique to CIU, or related to enhanced circulating basophil marker expression.<\/jats:p>","DOI":"10.1111\/j.1365-2222.2006.02494.x","type":"journal-article","created":{"date-parts":[[2006,6,1]],"date-time":"2006-06-01T21:56:18Z","timestamp":1149198978000},"page":"770-776","source":"Crossref","is-referenced-by-count":80,"title":["Evidence of <i>in vivo<\/i> basophil activation in chronic idiopathic urticaria"],"prefix":"10.1111","volume":"36","author":[{"given":"K.","family":"Vasagar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. 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