{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T16:01:49Z","timestamp":1769875309673,"version":"3.49.0"},"reference-count":32,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2006,6,1]],"date-time":"2006-06-01T00:00:00Z","timestamp":1149120000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2006,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:bold>Background\n\t\t\t\t\t<\/jats:bold> The relevant importance of individual allergens for allergic sensitization is only partially understood. More detailed information on allergen structure and how it influences immunological responses can lead to better diagnosis of disease and improved preparations for allergen\u2010specific immunotherapy. Grass pollen contains several different allergens, and although the group 3 allergens have been classified long ago, their structure and allergenicity have been poorly investigated.<\/jats:p><jats:p><jats:bold>Objective\n\t\t\t\t\t<\/jats:bold> To characterize Phl p 3 from timothy grass pollen and compare it with Phl p 2 with respect to biochemical structure and allergenicity.<\/jats:p><jats:p><jats:bold>Methods\n\t\t\t\t\t<\/jats:bold> Natural Phl p 2 and Phl p 3 were separated from a pollen extract by chromatography and characterized by 2D electrophoresis and protein sequencing. The complete sequences were determined by DNA cloning and detected in natural pollen extracts by mass spectrometry. Further comparisons of the allergens were made for IgE\u2010binding and cross\u2010reactivity, allergenicity was determined by basophil CD203c activation and skin prick test and 3D structures were compared by molecular modelling.<\/jats:p><jats:p><jats:bold>Results\n\t\t\t\t\t<\/jats:bold> Phl p 3 reveals molecular masses of 10.958 and 10.973\u2003kDa and <jats:italic>p<\/jats:italic>Is of 8.9 and 9.3, respectively, Phl p 2 a molecular mass of 10.816\u2003kDa and a <jats:italic>p<\/jats:italic>I of 4.6. The sequence identity is 58%. In spite of these differences in the primary structures, both allergens reveal similar conformational structures, resulting in similar immunological and allergological moieties.<\/jats:p><jats:p><jats:bold>Conclusions\n\t\t\t\t\t<\/jats:bold> The group 3 and group 2 allergens are major allergens with similar 3D structures. Although they differ considerably in their protein sequences and their <jats:italic>p<\/jats:italic>Is, they show only a slightly higher immunological reactivity for Phl p 3 on the B\u2010cell level (conformational epitopes). But distinct differences between the sequences may influence reactivity at the T cell level.<\/jats:p>","DOI":"10.1111\/j.1365-2222.2006.02505.x","type":"journal-article","created":{"date-parts":[[2006,6,1]],"date-time":"2006-06-01T21:56:18Z","timestamp":1149198978000},"page":"840-849","source":"Crossref","is-referenced-by-count":25,"title":["Phl p 3: Structural and immunological characterization of a major allergen of timothy grass pollen"],"prefix":"10.1111","volume":"36","author":[{"given":"Arnd","family":"Petersen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roland","family":"Suck","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Buko","family":"Lindner","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dessi","family":"Georgieva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Ernst","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Holger","family":"Notbohm","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Wicklein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oliver","family":"Cromwell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wolf\u2010Meinhard","family":"Becker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,6]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.04.00013904"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.1999.00653.x"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1159\/000069013"},{"key":"e_1_2_6_5_2","first-page":"8665","article-title":"Complete primary structure of a Lolium perenne (perennial rye grass) pollen allergen, Lol p III","volume":"28","author":"Ansari AA","year":"1989","journal-title":"comparison with known Lol p I and II sequences"},{"key":"e_1_2_6_6_2","unstructured":"MatthiesenF FribergL OlsenM LowensteinH.Purification and Characterization ofPhleum pratense(timothy) pollen allergenPhl pVI. 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