{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T21:57:26Z","timestamp":1773007046379,"version":"3.50.1"},"reference-count":40,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2006,9,4]],"date-time":"2006-09-04T00:00:00Z","timestamp":1157328000000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2006,9]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:bold>Background\n\t\t\t\t\t<\/jats:bold> Eosinophils are one of the major effector cells in bronchial asthma. Their infiltration of airways correlates with the asthma severity. Recruitment and activation of eosinophils are partially mediated by integrins \u03b1<jats:sub>4<\/jats:sub>\u03b2<jats:sub>1<\/jats:sub> and \u03b1<jats:sub>4<\/jats:sub>\u03b2<jats:sub>7<\/jats:sub>. Collagens type I and IV constitute important components of extracellular matrix and vascular basement membrane, respectively. Therefore, collagen\u2010binding integrins (\u03b1<jats:sub>1<\/jats:sub>\u03b2<jats:sub>1<\/jats:sub> and \u03b1<jats:sub>2<\/jats:sub>\u03b2<jats:sub>1<\/jats:sub>) may also play a role in eosinophil lung infiltration.<\/jats:p><jats:p><jats:bold>Objective\n\t\t\t\t\t<\/jats:bold> To evaluate the possible presence of \u03b1<jats:sub>1<\/jats:sub>\u03b2<jats:sub>1<\/jats:sub> and \u03b1<jats:sub>2<\/jats:sub>\u03b2<jats:sub>1<\/jats:sub> integrins on peripheral blood eosinophils from asthmatic subjects.<\/jats:p><jats:p><jats:bold>Methods\n\t\t\t\t\t<\/jats:bold> Collagen receptors were studied on eosinophils separated by immunomagnetic CD16\u2010negative method from healthy donors (<jats:italic>n<\/jats:italic>=13) and patients with moderate persistent atopic bronchial asthma (<jats:italic>n<\/jats:italic>=15). Surface receptor identification was performed by flow cytometry and cell adhesion assay.<\/jats:p><jats:p><jats:bold>Results\n\t\t\t\t\t<\/jats:bold> Eosinophils isolated from the patients showed increased expression of both \u03b1<jats:sub>1<\/jats:sub>\u03b2<jats:sub>1<\/jats:sub> and \u03b1<jats:sub>2<\/jats:sub>\u03b2<jats:sub>1<\/jats:sub> integrins as compared with healthy controls. Moreover, adhesive function of eosinophils to collagen type IV was inhibited by snake venom disintegrins: viperistatin and obtustatin. These disintegrins contain KTS active motif and are specific inhibitors of \u03b1<jats:sub>1<\/jats:sub>\u03b2<jats:sub>1<\/jats:sub> integrin.<\/jats:p><jats:p><jats:bold>Conclusion\n\t\t\t\t\t<\/jats:bold> We demonstrated for the first time that collagen receptors: \u03b1<jats:sub>1<\/jats:sub>\u03b2<jats:sub>1<\/jats:sub> and \u03b1<jats:sub>2<\/jats:sub>\u03b2<jats:sub>1<\/jats:sub> integrins are overexpressed on the surface of peripheral blood eosinophils of asthmatic subjects. Further studies may reveal potential application of KTS\u2010disintegrins or their structural analogs for therapy of bronchial asthma.<\/jats:p>","DOI":"10.1111\/j.1365-2222.2006.02540.x","type":"journal-article","created":{"date-parts":[[2006,9,4]],"date-time":"2006-09-04T16:26:22Z","timestamp":1157387182000},"page":"1184-1191","source":"Crossref","is-referenced-by-count":13,"title":["Increased expression of collagen receptors: \u03b1<sub>1<\/sub>\u03b2<sub>1<\/sub> and \u03b1<sub>2<\/sub>\u03b2<sub>1<\/sub> integrins on blood eosinophils in bronchial 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