{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T18:17:09Z","timestamp":1792088229175,"version":"4.4.0"},"reference-count":46,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2006,9,4]],"date-time":"2006-09-04T00:00:00Z","timestamp":1157328000000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2006,9]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:bold>Background\n\t\t\t\t\t<\/jats:bold> Transplacental transfer of nutritive and inhalant allergens has been described being potentially responsible for a series of events leading to antigen\u2010specific immune responses in the fetus. As such, cord blood T cell responses appear ubiquitously. However, studies failed to reveal a consistent dose\u2013response relationship between antenatal allergen exposure and allergen\u2010specific cellular reactivity in cord blood.<\/jats:p><jats:p><jats:bold>Objective\n\t\t\t\t\t<\/jats:bold> To examine the transfer process of allergens (ovalbumin (OVA), \u03b2\u2010lactoglobulin (BLG), birch pollen allergen Bet v1) in placental tissue (BeWo cell line, <jats:italic>ex vivo<\/jats:italic> placenta model).<\/jats:p><jats:p><jats:bold>Methods\n\t\t\t\t\t<\/jats:bold> The choriocarcinoma cell line BeWo was used to study the allergen uptake and transfer experiments <jats:italic>in vitro<\/jats:italic>. In the <jats:italic>ex vivo<\/jats:italic> placenta model the contribution of different placental compartments was evaluated. For this, immuno\u2010histochemistry, immuno\u2010electronmicroscopy and ELISA techniques were applied using monoclonal antibodies to Bet v1, OVA and \u2010BLG.<\/jats:p><jats:p><jats:bold>Results\n\t\t\t\t\t<\/jats:bold>  <jats:italic>In vitro<\/jats:italic> transfer studies on a BeWo cell\u2010layer revealed an intracellular allergen uptake and a trans\u2010trophoblastic allergen transfer, which was temperature\u2010 and concentration dependent, pH sensitive and asymmetric. Allergen\u2010specific staining of placental tissue after allergen perfusion (BLG) demonstrated bulk of the allergen in the syncytio\u2010trophoblastic cell layer and minor staining in the villous stroma and in the endothelium of fetal vessels. Immunogold staining revealed an accumulation of the perfused allergen in the trophoblastic basement membrane.<\/jats:p><jats:p><jats:bold>Conclusion\n\t\t\t\t\t<\/jats:bold>  <jats:italic>In vitro\/ex vivo<\/jats:italic> trans\u2010trophoblastic and trans\u2010placental allergen transfer is shown with an accumulation of most of the allergen in placental tissues, potentially explaining the missing direct dose\u2013response relationship between prenatal (maternal) allergen exposure and allergen\u2010specific cellular reactivity in cord blood.<\/jats:p>","DOI":"10.1111\/j.1365-2222.2006.02559.x","type":"journal-article","created":{"date-parts":[[2006,9,4]],"date-time":"2006-09-04T16:26:22Z","timestamp":1157387182000},"page":"1130-1137","source":"Crossref","is-referenced-by-count":29,"title":["Most of diaplacentally transferred allergen is retained in the 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