{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T18:18:13Z","timestamp":1770833893188,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2006,10,2]],"date-time":"2006-10-02T00:00:00Z","timestamp":1159747200000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2006,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:bold>Background\n\t\t\t\t\t<\/jats:bold> There is renewed interest in the role played by specific counter\u2010regulatory mechanisms to control the inflammatory host response, poorly investigated in human pathology. Here, we monitored the expression of two anti\u2010inflammatory mediators, annexin 1 and galectin\u20101, and assessed their potential link to glucocorticoids' (GCs) effective control of nasal polyposis (NP).<\/jats:p><jats:p><jats:bold>Methods\n\t\t\t\t\t<\/jats:bold> Total patterns of mRNA and protein expression were analysed by quantitative real\u2010time PCR (qPCR) and Western blotting analyses, whereas ultrastructural immunocytochemistry was used for spatial localization and quantification of each mediator, focusing on mast cells, eosinophils and epithelial cells.<\/jats:p><jats:p><jats:bold>Results\n\t\t\t\t\t<\/jats:bold> Up\u2010regulation of the annexin 1 gene, and down\u2010regulation of galectin\u20101 gene, was detected in polypoid tissue compared with nasal mucosa. Patient treatment with betamethasone augmented galectin\u20101 protein expression in polyps. At the cellular level, control mast cells and eosinophils displayed higher annexin 1 expression, whereas marked galectin\u20101 immunolabelling was detected in the granule matrix of mast cells. Cells of glandular duct epithelium also displayed expression of both annexin 1 and galectin\u20101, augmented after treatment.<\/jats:p><jats:p><jats:bold>Conclusion\n\t\t\t\t\t<\/jats:bold> Mast cells and epithelial cells appeared to be pivotal cell types involved in the expression of both annexin 1 and galectin\u20101. It is possible that annexin 1 and galectin\u20101 could be functionally associated with a specific mechanism in NP and that GC exert at least part of their beneficial effects on the airway mucosa by up\u2010regulating, in a specific cell target fashion, these anti\u2010inflammatory agonists.<\/jats:p>","DOI":"10.1111\/j.1365-2222.2006.02570.x","type":"journal-article","created":{"date-parts":[[2006,10,2]],"date-time":"2006-10-02T14:33:17Z","timestamp":1159799597000},"page":"1260-1267","source":"Crossref","is-referenced-by-count":12,"title":["Spatial expression of two anti\u2010inflammatory mediators, annexin 1 and galectin\u20101, in nasal polyposis"],"prefix":"10.1111","volume":"36","author":[{"given":"A. A. S.","family":"Sena","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. J. S.","family":"Provazzi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. M.","family":"Fernandes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. M.","family":"Cury","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P.","family":"Rahal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. M.","family":"Oliani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,10,2]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11882-002-0088-9"},{"key":"e_1_2_5_3_2","first-page":"1","article-title":"Nasal polyposis","volume":"3","author":"Pawankar R.","year":"2003","journal-title":"an update editorial review"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.1998.tb03767.x"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1177\/000348949910800612"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1159\/000087639"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.130.2.211"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3780407"},{"key":"e_1_2_5_9_2","first-page":"331","article-title":"Annexins","volume":"82","author":"Gerke V","year":"2002","journal-title":"from structure to function"},{"key":"e_1_2_5_10_2","first-page":"60","article-title":"Annexin 1","volume":"18","author":"Perretti M","year":"2003","journal-title":"an endogenous anti-inflammatory protein"},{"key":"e_1_2_5_11_2","first-page":"125","article-title":"Annexin 1","volume":"53","author":"Parente L","year":"2004","journal-title":"more than an anti-phospholipase protein"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4827(03)00310-0"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(90)91445-G"},{"key":"e_1_2_5_14_2","first-page":"605","article-title":"Measurement of lipocortin 1 levels in murine peripheral blood leukocytes by flow cytometry","volume":"118","author":"Perretti M","year":"1996","journal-title":"modulation by glucocorticoids and inflammation"},{"key":"e_1_2_5_15_2","first-page":"565","article-title":"Shedding light on the immunomodulatory properties of galectins","volume":"19","author":"Rabinovich GA","year":"2004","journal-title":"novel regulators of innate and adaptive immune responses"},{"key":"e_1_2_5_16_2","first-page":"1429","article-title":"An immunocytochemical and in situ hibridization analysis of annexin 1 expression in rat mast cells","volume":"80","author":"Oliani SM","year":"2000","journal-title":"modulation by inflammation and dexamethasone"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64002-3"},{"key":"e_1_2_5_18_2","doi-asserted-by":"publisher","DOI":"10.1080\/09629359791505"},{"key":"e_1_2_5_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(90)90241-M"},{"key":"e_1_2_5_20_2","doi-asserted-by":"crossref","first-page":"1405","DOI":"10.1242\/jcs.111.10.1405","article-title":"Lipocortin 1 (annexin 1) in patches associated with the membrane of a lung adenocarcinoma cell line and in the cell cytoplasm","volume":"111","author":"Traverso V","year":"1998","journal-title":"J Cell Sci"},{"key":"e_1_2_5_21_2","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb\/6.1.17"},{"key":"e_1_2_5_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1196-1259"},{"key":"e_1_2_5_23_2","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2001.112374"},{"key":"e_1_2_5_24_2","first-page":"257","article-title":"Distribution of mast cells and eosinophils in nasal polyps from atopic and nonatopic subjects","volume":"11","author":"Pawliczak R","year":"1997","journal-title":"a morphometric study"},{"key":"e_1_2_5_25_2","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.1997.01900060037006"},{"key":"e_1_2_5_26_2","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v10.i10.1466"},{"key":"e_1_2_5_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.510240114"},{"key":"e_1_2_5_28_2","doi-asserted-by":"publisher","DOI":"10.1165\/ajrcmb.18.1.2808"},{"key":"e_1_2_5_29_2","first-page":"1914","article-title":"Expression of galectins\u20101 and \u20103 correlates with defective mononuclear cell apoptosis in patients with juvenile idiopathic arthritis","volume":"28","author":"Harjacek M","year":"2001","journal-title":"J Rheumatol"},{"key":"e_1_2_5_30_2","first-page":"193","article-title":"Topical glucocorticoids inhibit neurogenic inflammation","volume":"283","author":"Ahluwalia A","year":"1995","journal-title":"involvement of lipocortin 1"},{"key":"e_1_2_5_31_2","first-page":"147","article-title":"The microcirculation and inflammation","volume":"7","author":"Perretti M","year":"2000","journal-title":"site of action for glucocorticoids"},{"key":"e_1_2_5_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(94)90210-0"},{"key":"e_1_2_5_33_2","doi-asserted-by":"publisher","DOI":"10.1080\/09629350210000015683"},{"key":"e_1_2_5_34_2","first-page":"12","article-title":"Analysis of the annexin 1 expression in rat trachea","volume":"1","author":"Damazo AS","year":"2004","journal-title":"study of the mast cell heterogeneity"},{"key":"e_1_2_5_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjp.0703272"},{"key":"e_1_2_5_36_2","first-page":"467","article-title":"Galectin\u20101","volume":"19","author":"Scott K","year":"2004","journal-title":"a bifunctional regulator of cellular proliferation"}],"container-title":["Clinical &amp; Experimental Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2222.2006.02570.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2222.2006.02570.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T19:10:51Z","timestamp":1697224251000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2222.2006.02570.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,10]]},"references-count":35,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2006,10]]}},"alternative-id":["10.1111\/j.1365-2222.2006.02570.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2222.2006.02570.x","archive":["Portico"],"relation":{},"ISSN":["0954-7894","1365-2222"],"issn-type":[{"value":"0954-7894","type":"print"},{"value":"1365-2222","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,10]]}}}