{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T18:38:58Z","timestamp":1757702338127},"reference-count":18,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2006,11,2]],"date-time":"2006-11-02T00:00:00Z","timestamp":1162425600000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2006,11]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:bold>Background\n\t\t\t\t\t<\/jats:bold> Immunological oral tolerance is being studied with great interest due to its therapeutic potential in allergy and autoimmunity processes, although the cellular and molecular mechanisms linking these different phenomena remain elusive. In the present study, two mouse lines with extreme phenotypes for susceptibility [TS Line] or resistance [TR Line] to oral tolerance and their [TS \u00d7 TR]F<jats:sub>2<\/jats:sub> segregants were used in order to evaluate the impact of these traits on the atopic potential of the individuals.<\/jats:p><jats:p><jats:bold>Objective\n\t\t\t\t\t<\/jats:bold> Demonstrate whether the <jats:italic>tr<\/jats:italic> and <jats:italic>ts<\/jats:italic> genes, cumulated during 18 generations of bidirectional genetic selection, influence expression of two important immunobiological traits (IgE and mast cell) critical to allergic response.<\/jats:p><jats:p><jats:bold>Methods\n\t\t\t\t\t<\/jats:bold> Mice with extreme phenotypes for oral tolerance to ovalbumin (OVA), produced by assortative mating (TS and TR Line), and their (TS \u00d7 TR)F<jats:sub>2<\/jats:sub> segregating were used. Serum IgE levels assayed by ELISA, and mastocytes counted with toluidine blue staining were evaluated in na\u00efve mice. Anaphylaxis was induced by intravenous injection of OVA, intestinal inflammation by oral administration of OVA 7 days after immunization, and pulmonary inflammation by intranasal and nebulization OVA challenges. Specific IgE was dosed by passive cutaneous anaphylaxis.<\/jats:p><jats:p><jats:bold>Results\n\t\t\t\t\t<\/jats:bold> The na\u00efve TS mice have a 20\u2010fold lower serum IgE level and two\u2010 to threefold diminished mast cell numbers in mucosal sites, when compared with TR\u2010mice, which were highly susceptible to allergic inflammation and anaphylactic shock. The associations of oral tolerance, serum IgE levels and mast cell numbers in na\u00efve animals were confirmed analysing the simultaneous presence of these traits in individuals of a [TS \u00d7 TR]F<jats:sub>2<\/jats:sub>\u2010segregating population.<\/jats:p><jats:p><jats:bold>Conclusion\n\t\t\t\t\t<\/jats:bold> The results suggest that the complex of genes controlling TS and TR phenotypes play a main role in the regulation of the atopic potential of the individual. The studies of these traits in interline F<jats:sub>2<\/jats:sub> segregants demonstrated a co\u2010segregation of TS and TR phenotypes with IgE responsiveness and mast cell numbers. Thus, the opposite capacity of the genetically modified mice may be involved in co\u2010adaptative mechanisms reflecting a dynamic relation between gene frequencies in a natural population. These correlations give circumstantial evidence to support clinical applications of oral tolerance in allergic and autoimmune diseases.<\/jats:p>","DOI":"10.1111\/j.1365-2222.2006.02588.x","type":"journal-article","created":{"date-parts":[[2006,11,2]],"date-time":"2006-11-02T20:14:38Z","timestamp":1162498478000},"page":"1399-1407","source":"Crossref","is-referenced-by-count":6,"title":["Genetic selection for resistance or susceptibility to oral tolerance imparts correlation to both Immunoglobulin E level and mast cell number phenotypes with a profound impact on the atopic potential of the individual"],"prefix":"10.1111","volume":"36","author":[{"given":"M. F. S.","family":"Da Silva","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"N\u00f3brega","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R. 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