{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T19:33:20Z","timestamp":1790969600533,"version":"4.1.0"},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,12,20]],"date-time":"2006-12-20T00:00:00Z","timestamp":1166572800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2007,1]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:bold>Background\n\t\t\t\t\t<\/jats:bold> Recognition of specific peanut allergens or the diversity of IgE binding to peanut allergens may play a role in the elicitation of severe allergic reactions.<\/jats:p><jats:p><jats:bold>Objective\n\t\t\t\t\t<\/jats:bold> To investigate whether sensitization to individual allergens Ara h 1, Ara h 2, Ara h 3 and Ara h 6 is correlated with clinical severity.<\/jats:p><jats:p><jats:bold>Methods\n\t\t\t\t\t<\/jats:bold> The reactivity of purified peanut allergens was measured by skin prick test (SPT) and by IgE immunoblot in 30 patients. The results were related to the clinical reactivity by history, and in 25 of them to the eliciting dose (ED).<\/jats:p><jats:p><jats:bold>Results\n\t\t\t\t\t<\/jats:bold> The majority of patients recognized Ara h 2 and Ara h 6. Patients with severe symptoms had a higher SPT response to Ara h 2 and Ara h 6 at low concentrations (0.1\u2003\u03bcg\/mL) and to Ara h 1 and Ara h 3 at higher concentrations (100\u2003\u03bcg\/mL), compared with patients with mild symptoms. They also recognized a greater number of allergens and showed a higher cumulative SPT response compared with patients with mild symptoms. No significant differences were observed between patients with a low or high ED.<\/jats:p><jats:p><jats:bold>Conclusions\n\t\t\t\t\t<\/jats:bold> Ara h 2 and Ara h 6 appeared to be more potent than Ara h 1 and Ara h 3. Both SPT reactivity to low concentrations of Ara h 2 and Ara h 6 and to higher concentrations of Ara h 1 and Ara h 3 were shown to be indicative of severe symptoms.<\/jats:p>","DOI":"10.1111\/j.1365-2222.2006.02628.x","type":"journal-article","created":{"date-parts":[[2006,12,21]],"date-time":"2006-12-21T07:50:20Z","timestamp":1166687420000},"page":"108-115","source":"Crossref","is-referenced-by-count":115,"title":["Does skin prick test reactivity to purified allergens correlate with clinical severity of peanut allergy?"],"prefix":"10.1111","volume":"37","author":[{"given":"K. A. B. M.","family":"Peeters","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. J.","family":"Koppelman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E.","family":"Van Hoffen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. W. H.","family":"Van Der Tas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. F.","family":"Den Hartog Jager","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. H.","family":"Penninks","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. L.","family":"Hefle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. A. F. M.","family":"Bruijnzeel\u2010Koomen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. F.","family":"Knol","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. C.","family":"Knulst","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,12,20]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"669","article-title":"Food hypersensitivity and atopic dermatitis","volume":"107","author":"Sampson HA","year":"1985","journal-title":"evaluation of 113 patients"},{"key":"e_1_2_6_3_2","first-page":"784","article-title":"Rising prevalence of allergy to peanut in children","volume":"110","author":"Grundy J","year":"2002","journal-title":"data from 2 sequential cohorts"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1398-9995.1999.00768.x"},{"key":"e_1_2_6_5_2","first-page":"1203","article-title":"Prevalence of peanut and tree nut allergy in the United States determined by means of a random digit dial telephone survey","volume":"112","author":"Sicherer SH","year":"2003","journal-title":"a 5-year follow-up study"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2002.129235"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199208063270603"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2001.112031"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2005.02.011"},{"key":"e_1_2_6_10_2","first-page":"583","article-title":"Relevance of Ara h1, Ara h2 and Ara h3 in peanut\u2010allergic patients, as determined by immunoglobulin E Western blotting, basophil\u2010histamine release and intracutaneous testing","volume":"34","author":"Koppelman SJ","year":"2004","journal-title":"Ara h2 is the most important peanut allergen"},{"key":"e_1_2_6_11_2","first-page":"490","article-title":"Purification and immunoglobulin E\u2010binding properties of peanut allergen Ara h 6","volume":"35","author":"Koppelman SJ","year":"2005","journal-title":"evidence for cross-reactivity with Ara h 2"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.1998.tb03967.x"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.1998.00386.x"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2004.01.779"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1081-1206(10)61306-3"},{"key":"e_1_2_6_16_2","first-page":"390","article-title":"Isolation and characterization of natural Ara h 6","volume":"48","author":"Suhr M","year":"2004","journal-title":"evidence for a further peanut allergen with putative clinical relevance based on resistance to pepsin digestion and heat"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1159\/000024203"},{"key":"e_1_2_6_18_2","first-page":"141","article-title":"Recombinant lipid transfer protein Cor a 8 from hazelnut","volume":"113","author":"Schocker F","year":"2004","journal-title":"a new tool for in vitro diagnosis of potentially severe hazelnut allergy"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1398-9995.1998.tb04965.x"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2005.02252.x"},{"key":"e_1_2_6_21_2","first-page":"776","article-title":"Microarray immunoassay","volume":"113","author":"Shreffler WG","year":"2004","journal-title":"association of clinical history, in vitro IgE function, and heterogeneity of allergenic peanut epitopes"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2005.06.033"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.3109\/02770906609106941"},{"key":"e_1_2_6_24_2","first-page":"689","article-title":"A consensus protocol for the determination of the threshold doses for allergenic food","volume":"34","author":"Taylor SL","year":"2004","journal-title":"how much is too much?"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2005.11.035"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.1998.00301.x"},{"key":"e_1_2_6_27_2","first-page":"1144","article-title":"Peanut allergen Ara h 3","volume":"58","author":"Koppelman SJ","year":"2003","journal-title":"isolation from peanuts and biochemical characterization"},{"issue":"8","key":"e_1_2_6_28_2","first-page":"38","article-title":"Skin tests in the diagnosis of food allergy","volume":"6","author":"Dreborg S.","year":"1995","journal-title":"Pediatr Allergy Immunol"},{"key":"e_1_2_6_29_2","first-page":"61","article-title":"Precise area determination of skin\u2010prick tests","volume":"23","author":"Poulsen LK","year":"1993","journal-title":"validation of a scanning device and software for a personal computer"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2005.07.004"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1398-9995.2001.056002132.x"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2005.02312.x"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1067\/mai.2003.1621"},{"key":"e_1_2_6_34_2","first-page":"596","article-title":"An evaluation of the sensitivity of subjects with peanut allergy to very low doses of peanut protein","volume":"100","author":"Hourihane JO'B","year":"1997","journal-title":"a randomized, double-blind, placebo-controlled food challenge study"}],"container-title":["Clinical &amp; Experimental Allergy"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fj.1365-2222.2006.02628.x","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/j.1365-2222.2006.02628.x","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T23:18:59Z","timestamp":1697239139000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/j.1365-2222.2006.02628.x"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,12,20]]},"references-count":33,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2007,1]]}},"alternative-id":["10.1111\/j.1365-2222.2006.02628.x"],"URL":"https:\/\/doi.org\/10.1111\/j.1365-2222.2006.02628.x","archive":["Portico"],"relation":{},"ISSN":["0954-7894","1365-2222"],"issn-type":[{"value":"0954-7894","type":"print"},{"value":"1365-2222","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,12,20]]}}}