{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T08:14:30Z","timestamp":1791274470640,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,12,20]],"date-time":"2006-12-20T00:00:00Z","timestamp":1166572800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clin Experimental Allergy"],"published-print":{"date-parts":[[2007,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:bold>Background\n\t\t\t\t\t<\/jats:bold> Microbial intestinal colonization in early in life is regarded to play a major role for the maturation of the immune system. Application of non\u2010pathogenic probiotic bacteria during early infancy might protect from allergic disorders but underlying mechanisms have not been analysed so far.<\/jats:p><jats:p><jats:bold>Objective\n\t\t\t\t\t<\/jats:bold> The aim of the current study was to investigate the immune effects of oral application of probiotic bacteria on allergen\u2010induced sensitization and development of airway inflammation and airway hyper\u2010reactivity, cardinal features of bronchial asthma.<\/jats:p><jats:p><jats:bold>Methods\n\t\t\t\t\t<\/jats:bold> Newborn Balb\/c mice received orally 10<jats:sup>9<\/jats:sup> CFU every second day either <jats:italic>Lactobacillus rhamnosus GG<\/jats:italic> or <jats:italic>Bifidobacterium lactis<\/jats:italic> (Bb\u201012) starting from birth for consecutive 8 weeks, during systemic sensitization (six intraperitoneal injections, days 29\u201340) and airway challenge (days 54\u201356) with ovalbumin.<\/jats:p><jats:p><jats:bold>Results\n\t\t\t\t\t<\/jats:bold> The administration of either Bb\u201012 or LGG suppressed all aspects of the asthmatic phenotype: airway reactivity, antigen\u2010specific immunoglobulin E production and pulmonary eosinophilia (mean: 137 vs. 17 and 13 cells \u00d7 10<jats:sup>3<\/jats:sup>\/mL, respectively). Antigen\u2010specific recall proliferation by spleen cells and T\u2010helper type 2 cytokine production (IL\u20104, IL\u20105 and IL\u201010) by mesenteric lymph node cells also showed significant reduction, while TGF production remained unchanged. Oral LGG administration particularly suppressed allergen\u2010induced proliferative responses and was associated with an increase in numbers of TGF\u2010\u03b2\u2010secreting CD4<jats:sup>+<\/jats:sup>\/CD3<jats:sup>+<\/jats:sup> T cells in mesenteric lymph nodes (6.5, 16.7%) as well as nearly 2\u2010fold up\u2010regulation of Foxp3\u2010expressing cells in peribronchial lymph nodes.<\/jats:p><jats:p><jats:bold>Conclusions\n\t\t\t\t\t<\/jats:bold> Neonatal application of probiotic bacteria inhibits subsequent allergic sensitization and airway disease in a murine model of asthma by induction of T regulatory cells associated with increased TGF\u2010\u03b2 production.<\/jats:p>","DOI":"10.1111\/j.1365-2222.2006.02629.x","type":"journal-article","created":{"date-parts":[[2006,12,21]],"date-time":"2006-12-21T07:50:05Z","timestamp":1166687405000},"page":"498-505","source":"Crossref","is-referenced-by-count":279,"title":["Probiotic\u2010induced suppression of allergic sensitization and airway inflammation is associated with an increase of T regulatory\u2010dependent mechanisms in a murine model of asthma"],"prefix":"10.1111","volume":"37","author":[{"given":"W.","family":"Feleszko","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Jaworska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R\u2010D.","family":"Rha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Steinhausen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Avagyan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Jaudszus","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Ahrens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. 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